Anti-Asthmatic Effects of Saffron Extract and Salbutamol in an Ovalbumin-Induced Airway Model of Allergic Asthma
| Autor: | Kedar S. Prabhavalkar, Pranav Nair |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
saffron Pharmacology 03 medical and health sciences 0302 clinical medicine Immune system medicine IL’s Asthma Lung medicine.diagnostic_test biology business.industry Albumin ovalbumin General Medicine asthma respiratory system lcsh:Otorhinolaryngology medicine.disease lcsh:RF1-547 respiratory tract diseases Ovalbumin 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure 030228 respiratory system salbutamol TNF-α biology.protein Salbutamol business Airway medicine.drug |
| Zdroj: | Sinusitis Volume 5 Issue 1 Pages 3-31 Sinusitis, Vol 5, Iss 3, Pp 17-31 (2021) |
| ISSN: | 2673-351X |
| DOI: | 10.3390/sinusitis5010003 |
| Popis: | Introduction: Asthma is a chronic inflammatory disorder of the airways often characterized by airway remodeling and influx of inflammatory cells into the airways. Saffron (C. sativus) has been reported to possess anti-inflammatory, anti-allergic and immunomodulatory properties. Salbutamol is known to relax airway smooth muscles. Objective: To investigate the combined anti-asthmatic effect of C. sativus extract (CSE) and salbutamol in an ovalbumin (OVA)-induced asthma in rats. Materials and methods: Airway hyperresponsiveness (AHR) was induced in male Sprague-Dawley rats by OVA challenge and treated with CSE (30 mg/kg and 60 mg/kg i.p.) and salbutamol (0.5 mg/kg p.o) for 28 days. After the induction period, various hematological, biochemical, molecular (ELISA) and histological analyses were performed. Results: OVA-induced alterations observed in hematological parameters (total and differential cell counts observed in Bronchoalveolar Lavage Fluid (BALF) were significantly attenuated (p < 0.01) by CSE (30 mg/kg and 60 mg/kg) and salbutamol (0.5 mg/kg). The treatment combination also significantly decreased (p < 0.01) the levels of total protein and albumin in serum, BALF and lung tissues. Treatment with CSE and salbutamol significantly attenuated (p < 0.01) increase in OVA induced Th2 cytokine levels (TNF-&alpha IL-1&beta IL-4, IL-13). Histopathological analysis of lung tissue showed that combined effect of CSE and salbutamol treatment ameliorated OVA-induced inflammatory influx and ultrastructural aberrations. Conclusion: The results obtained from this study show that the combined effect of CSE and salbutamol exhibited anti-asthmatic properties via its anti-inflammatory effect and by alleviating Th2 mediated immune response. Thus, this treatment combination could be considered as a new therapeutic strategy for management of asthma. |
| Databáze: | OpenAIRE |
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