Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Cultured on Magnesium Oxide/Polycaprolactone Nanofibrous Scaffolds for Improving Bone Tissue Reconstruction
| Autor: | Hakimeh Zali, Parviz Ranjbarvan, Mostafa Rezaei Tavirani, Meisam Omidi, Vahid Mansouri, Zahra Niknam, Ali Golchin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Magnesium
Mesenchymal stem cell Pharmaceutical Science chemistry.chemical_element RM1-950 Bone tissue magnesium oxide Electrospinning osteogenesis chemistry.chemical_compound medicine.anatomical_structure Tissue engineering chemistry polycaprolactone Nanofiber tissue engineering Polycaprolactone medicine Alkaline phosphatase adipose-derived stem cells Therapeutics. Pharmacology General Pharmacology Toxicology and Pharmaceutics electrospinning Biomedical engineering |
| Zdroj: | Advanced Pharmaceutical Bulletin, Vol 12, Iss 1, Pp 142-154 (2022) |
| ISSN: | 2251-7308 2228-5881 |
| Popis: | Purpose: Recently, bone tissue engineering as a new strategy is used to repair and replace bone defects due to limitations in allograft and autograft methods. In this regard, we prepared nanofibrous scaffolds composed of polycaprolactone and magnesium oxide nanoparticles using the electrospinning technique for possible bone tissue engineering applications. Methods: The fabricated composites were characterized via scanning electron microscopy imaging of scaffolds and seeded cells, water contact angle, DAPI staining, and MTT assay. Then osteogenic differentiation of adipose-derived mesenchymal stem cells cultured on this composite scaffold was determined by standard osteogenic marker tests, including alkaline phosphatase activity, calcium deposition, and expression of osteogenic differentiation genes in the laboratory conditions. Results: The Scanning electron microscopy analysis demonstrated that the diameter of nanofibers significantly decreased from 1029.25±209.349 µm to 537.83+0.140 nm, with the increase of MgO concentration to 2% (p |
| Databáze: | OpenAIRE |
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