Silver Nanoparticles and Metallic Silver Interfere with the Griess Reaction: Reduction of Azo Dye Formation via a Competing Sandmeyer-Like Reaction
| Autor: | Rita La Spina, Agnieszka Kinsner-Ovaskainen, Douglas Gilliland, Angela A.M. Kämpfer, Sandro Valzacchi, David Asturiol, Vicki Stone |
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| Rok vydání: | 2017 |
| Předmět: |
Silver
Metal Nanoparticles Nanotechnology 02 engineering and technology Nitric Oxide 010402 general chemistry Toxicology medicine.disease_cause 01 natural sciences Mass Spectrometry Silver nanoparticle Cell Line Nitric oxide Nanomaterials Metal chemistry.chemical_compound Griess test medicine Sandmeyer reaction Humans Nitrite Coloring Agents Chromatography High Pressure Liquid Nitrites General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry visual_art visual_art.visual_art_medium 0210 nano-technology Azo Compounds Oxidation-Reduction Genotoxicity Nuclear chemistry |
| Zdroj: | Chemical Research in Toxicology. 30:1030-1037 |
| ISSN: | 1520-5010 0893-228X |
| DOI: | 10.1021/acs.chemrestox.6b00280 |
| Popis: | Silver (Ag) is the most common nanomaterial (NM) in consumer products. Much research has been focused on elucidating the potential impact of Ag-containing NMs on human health, e.g., cytotoxicity, genotoxicity, or proinflammatory responses. In the case of proinflammatory responses, a frequently used end point is the induction of nitric oxide (NO), which is indirectly quantified as nitrite (NO2–) with the Griess reaction. After preliminary studies in a macrophage-like cell culture system showed anomalous false negative results in the presence of silver nanoparticles (Ag NPs), we studied the influence of Ag on the detection of NO2– in a cell-free environment. Solutions containing a known concentration of NaNO2 were prepared in H2O, PBS, or complete cell culture medium (CCM) and analyzed using the Griess reaction in the presence of Ag in its metallic or ionic state. In Milli-Q H2O, the impact of salts on the detection was investigated using NaCl and KBr. After completion of the Griess reaction, the samples we... |
| Databáze: | OpenAIRE |
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