Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis

Autor: Hirotoshi Fujita, Kazumichi Kawakubo, Atsushi Masamune, Hiroshi Takeda, Shunsuke Ohnishi, Masaki Kuwatani, Reizo Onishi, Naoya Sakamoto
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Lipopolysaccharides
Pathology
Endocrinology
Diabetes and Metabolism
Pancreatic stellate cell
Extraembryonic Membranes
Acinar Cells
Pharmacology
Rats
Sprague-Dawley
Endocrinology
Cells
Cultured
Chemokine CCL2
fetal membrane
amnion
Reverse Transcriptase Polymerase Chain Reaction
Pancreatic Stellate Cells
macrophages
medicine.anatomical_structure
Acute Disease
Amylases
Acute pancreatitis
Tumor necrosis factor alpha
Female
Pancreas
medicine.medical_specialty
acute pancreatitis
Transplantation
Heterologous
Mesenchymal Stem Cell Transplantation
Cell Line
chronic pancreatitis
03 medical and health sciences
Pancreatitis
Chronic
Internal Medicine
medicine
Acinar cell
Animals
Humans
mesenchymal stem cells
Hepatology
business.industry
Tumor Necrosis Factor-alpha
Mesenchymal stem cell
Macrophage Activation
medicine.disease
Coculture Techniques
Transplantation
030104 developmental biology
Pancreatitis
Culture Media
Conditioned
business
Zdroj: Pancreas : Journal of Neuroendocrine Tumors and Pancreatic Diseases and Sciences. 45(5):707-713
ISSN: 0885-3177
Popis: Objectives Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine and can be isolated from fetal membranes (FMs), particularly amniotic membranes. We investigated the effect of rat FM-derived MSCs (rFM-MSCs) and human amnion-derived MSCs (hAMSCs) on the inflammatory reaction in vitro and therapeutic effects in rats with acute and chronic pancreatitis. Methods Effect of rFM-MSCs or hAMSC-conditioned medium was investigated in vitro. Acute pancreatitis was induced by intraductal injection of 4% taurocholate, and rFM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and hAMSCs were transplanted intravenously. Results The inflammatory reaction of macrophages induced by lipopolysaccharide and trypsin was significantly suppressed by rFM-MSC coculture. Pancreatic acinar cell injury induced by cerulein was significantly ameliorated by hAMSC-conditioned medium. Pancreatic stellate cell activation induced by tumor necrosis factor-α was significantly decreased by hAMSC-conditioned medium. Transplantation of rFM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. The hAMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas. Conclusions Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats.
Databáze: OpenAIRE
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