IL-4 enhances IEC-6 intestinal epithelial cell proliferation yet has no effect on IL-6 secretion
| Autor: | S. J. D. Vitkus, D. W. Mcgee |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_treatment
Immunology Biology digestive system Epithelium Proinflammatory cytokine Cell Line Intestinal mucosa parasitic diseases medicine Immunology and Allergy Animals Secretion Interleukin 4 Cell growth Interleukin-6 Epithelial Cells Original Articles digestive system diseases Recombinant Proteins Rats Intestines Cytokine medicine.anatomical_structure Cell culture Interleukin-4 tissues Cell Division |
| Zdroj: | Clinical and experimental immunology. 105(2) |
| ISSN: | 0009-9104 |
| Popis: | Intestinal epithelial cells (IEC) form an important line of defence at the intestinal mucosa by providing a barrier to lumenal contents and also by their ability to secrete various inflammatory cytokines. Recently, several T cell-derived cytokines have been shown to regulate specific IEC functions. In this study, the effect of IL-4 on IEC proliferation and secretion of the inflammatory cytokine IL-6 was investigated using the non-transformed rat IEC-6 intestinal epithelial cell line. Recombinant rat (rr)IL-4 was found to enhance IEC-6 cell proliferation over 4 days of culture, and this enhancement was dose-dependent. Further studies using specific antibodies confirmed that IL-4 induced the effect and that the effect was not mediated by autocrine-produced transforming growth factor-alpha. However, IL-4 did not induce IL-6 secretion by the IEC-6 cells, nor did it alter IL-1β-induced IL-6 secretion. These results indicate that T cells may be capable of regulating IEC proliferation via the secretion of IL-4 without altering the capacity of the IEC to function in the inflammatory response by secreting IL-6. |
| Databáze: | OpenAIRE |
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