Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract
| Autor: | Damla Uludag, Derya Çiçek Polat, Mehmet Evren Okur, Ayşe Esra Karadağ, Nihal Karakaş |
|---|---|
| Přispěvatelé: | Anadolu Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Opuntia ficus-indica
ABTS Antioxidant antioxidant Traditional medicine Farmakoloji ve Eczacılık Chemistry DPPH medicine.medical_treatment Extract Pharmaceutical Science food and beverages Bain Cancer Cell Line Pharmacy chemistry.chemical_compound Cell culture Extract Opuntia ficus-indica Brain cancer cell line Colon cancer cell line Antioxidant Cancer cell brain cancer cell line medicine Cytotoxic T cell Viability assay Pharmacology and Pharmacy Quercetin colon cancer cell line |
| Zdroj: | Volume: 49, Issue: 3 154-160 İstanbul Journal of Pharmacy |
| ISSN: | 2587-2087 |
| Popis: | okur, mehmet evren/0000-0001-7706-6452 WOS: 000511129700007 Opuntia ficus-indica fruits are claimed to possess several health-beneficial properties. This study was carried out to determine the phytochemicals as well as to compare antioxidant activity and cell viability of the methanol extract of OFI-fruits. Antioxidant activity was evaluated by various in vitro tests such as DPPH and ABTS. LC-MS and HPLC analyses were then performed. the activity of the OFI-fruit extract on viability of U87-MG (glioblastoma multiform), HT-29 (colon cancer) and human primary fibroblast cells was investigated by determining viable cells on a luciferase based system (Cell TiterGlo; Promega; USA). the results showed that OFI-fruits were a source of antioxidant compounds. Moreover, the extract was found to have rich phenolic-flavonoid contents. Quercetin was found in the extract according to LC-MS and HPLC studies. A remarkable increased cell death in the HT-29 and U87 cell lines was detected in a concentration-dependent manner (1-10mg/mL). Among these cell lines, HT-29 responded to OFI-fruit treatment more sensitively to even the lowest dose (1mg/mL). the results of this study showed that the OFI-fruit has significant antioxidant capacity and leads to strong cytotoxic activity on various cancer cells. Therefore, it can be suggested that OFI-fruits may have chemo-preventive potential and can be studied further to clarify cytotoxic abilities. |
| Databáze: | OpenAIRE |
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