Allograft inflammatory factor 1 is a regulator of transcytosis in M cells
| Autor: | Seiji Nakamura, Shigeo Uchino, Shinichi Kohsaka, Shintaro Sato, Satoshi Kaneto, Hiroshi Kiyono, Sari Kishikawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Science General Physics and Astronomy Cell Count General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Antigen Animals Antigens education Antigen-presenting cell Yersinia enterocolitica Cell Shape Immunity Mucosal Microfold cell education.field_of_study Multidisciplinary CD40 biology Integrin beta1 Calcium-Binding Proteins Cell Membrane Microfilament Proteins Epithelial Cells General Chemistry biology.organism_classification Yersinia Cell biology Immunoglobulin A Mice Inbred C57BL Lactobacillus 030104 developmental biology Enterocytes Transcytosis Bacterial Translocation Allograft inflammatory factor 1 biology.protein Antibody |
| Zdroj: | Nature Communications Nature Communications, Vol 8, Iss 1, Pp 1-10 (2017) |
| ISSN: | 2041-1723 |
| Popis: | M cells in follicle-associated epithelium (FAE) are specialized antigen-sampling cells that take up intestinal luminal antigens. Transcription factor Spi-B regulates M-cell maturation, but the molecules that promote transcytosis within M cells are not fully identified. Here we show that mouse allograft inflammatory factor 1 (Aif1) is expressed by M cells and contributes to M-cell transcytosis. FAE in Aif1−/− mice has suppressed uptake of particles and commensal bacteria, compared with wild-type mice. Translocation of Yersinia enterocolitica, but not of Salmonella enterica serovar Typhimurium, leading to the generation of antigen-specific IgA antibodies, is also diminished in Aif1-deficient mice. Although β1 integrin, which acts as a receptor for Y. enterocolitica via invasin protein, is expressed on the apical surface membranes of M cells, its active form is rarely found in Aif1−/− mice. These findings show that Aif1 is important for bacterial and particle transcytosis in M cells. M cells are intestinal epithelial cells that are specialized to transcytose antigens and bacteria from the intestinal lumen to antigen presenting cells on the other side. Here the authors show that the actin-binding protein Aif1 is highly expressed by intestinal M cells and regulates this transcytosis. |
| Databáze: | OpenAIRE |
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