Sox10 regulates skin melanocyte proliferation by activating the DNA replication licensing factor MCM5
| Autor: | Zhongyuan Su, Jia Qu, Xiaozi Zheng, Shanpu Zhu, Ling Hou, Fan Lu, Xiaobo Zhang, Yipin Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
DNA Replication
0301 basic medicine Cell type Skin Neoplasms SOX10 Fluorescent Antibody Technique Cell Cycle Proteins Dermatology Melanocyte Biology Biochemistry Mice 03 medical and health sciences 0302 clinical medicine Hair cycle Melanoblast medicine Animals Humans Cell Lineage RNA Small Interfering Promoter Regions Genetic Melanoma Molecular Biology Cells Cultured Cell Proliferation Gene knockdown SOXE Transcription Factors Cell growth Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Neural Crest Gene Knockdown Techniques 030220 oncology & carcinogenesis embryonic structures Cancer research Melanocytes RNA Interference Melanocyte proliferation |
| Zdroj: | Journal of Dermatological Science. 85:216-225 |
| ISSN: | 0923-1811 |
| DOI: | 10.1016/j.jdermsci.2016.12.002 |
| Popis: | Background The control of cell proliferation is a fundamental aspect of tissue formation in development and regeneration. A cell type that illustrates this point particularly well is the neural crest-derived melanocyte, the pigment cell of vertebrates, as melanocytes can be followed easily during development and their pigment is directly visible in the integument of the adult. In mammals, melanocytes undergo physiological cycles of loss and proliferative regeneration during the hair cycle, and their proliferation is also critical during wound healing, repigmentation of depigmented lesions, and in melanoma formation and progression. Hence, a thorough analysis of the molecular parameters controlling melanocyte proliferation is crucial for our understanding of the physiology of this cell type both in health and disease. Objective SOX10 is a critical regulator in melanocytes and melanoma cells, but its specific role in their proliferation is far from clear. In this study we analyze the role of SOX10 in regulating mammalian melanocyte proliferation in a mouse model. Methods The role of SOX10 in melanoblast proliferation was analyzed in Sox10/+ mice by co-staining for melanocyte-specific markers and cell proliferation. In vitro, the role of SOX10 was studied by manipulating its levels using RNAi and analyzing the effects on DNA synthesis and cell growth and on gene expression at the RNA and protein levels. Results Reduction of Sox10 gene dose led to a reduction in the number of melanoblasts. Knockdown of Sox10 in melanocytes led to inhibition of cell proliferation and a decrease in the expression of the minichromosome maintenance complex component 5 (MCM5). In fact, SOX10 directly activated MCM5 transcription by binding to conserved SOX10 consensus DNA sequences in the MCM5 promoter. Furthermore, the defect in cell proliferation could be rescued partially by overexpression of MCM5 in Sox10 knockdown melanocytes. Conclusion The results suggest that the SOX10-MCM5 axis plays an important role in controlling melanocyte proliferation. Our findings provide novel insights into the regulatory mechanisms of melanocyte proliferation and may have implications for our understanding of the roles of SOX10 and MCM5 in abnormal melanocyte proliferation disorders such as cutaneous melanoma. |
| Databáze: | OpenAIRE |
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