Metagenomic analysis of black-stained plaques in permanent dentition
| Autor: | Dilek Tağtekin, Can Ilgin, Sema Sirma Ekmekci, Funda Yanıkoğlu, Halil Çelik, Neslihan Abaci, Aris Cakiris, Zeynep Çelik |
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| Přispěvatelé: | İstinye Üniversitesi, Diş Hekimliği Fakültesi, Klinik Bilimler Bölümü, Celik, Zeynep Ceren, Celik, Halil, İstanbul Kent Üniversitesi, Fakülteler, Diş Hekimliği Fakültesi, Klinik Bilimler Bölümü, Yanıkoğlu, Funda |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Bioinformatics analysis Permanent dentition Dentistry mothur Next Generation Sequencing Dental Caries Icdas ii 03 medical and health sciences 0302 clinical medicine stomatognathic system Humans Medicine In patient General Dentistry Oral Microbiome Adult patients business.industry 030206 dentistry Cell Biology General Medicine Staining Black or African American Dentition Permanent stomatognathic diseases 030104 developmental biology Otorhinolaryngology Black Stain Metagenomics business Tooth |
| ISSN: | 0006-5978 |
| Popis: | Objectives: We aimed to determine the aetiologic agent responsible for black staining of permanent dentition using next-generation sequencing and determine the relationship between caries and black stains. Materials and methods: A total of 52 systemically healthy patients with black-stained and caries-free (n = 13), black-stained and carious (n = 13), black stain-free and caries-free (n = 13), and black stain-free and carious (n = 13) teeth were enrolled in the study. The International Caries Detection and Assessment System (ICDAS II) was used for caries classification. Between 08:00 and 10:00, supragingival plaque samples were collected after a minimum of 8–12 h of accumulation and DNA samples were isolated. The samples were processed using the ZymoBIOMICS™ Service. Bioinformatics analysis was performed using mothur at usegalaxy.org. Data were analysed statistically using the Pearson chi-square and Fisher tests. Results: The number of caries-free teeth (ICDAS 0, 1, and 2) was significantly higher in patients with black stains (p = 0.007).Capnocytophaga (4.8 %), Corynebacterium (3.9 %), and Neisseria (5.4 %) species were the most abundant among all black-stained plaques (carious and caries-free) (p < 0.05). Capnocytophaga (10.8 %), Cardiobacterium (3.6 %), and Rothia (1.72 %) species were detected in the black-stained plaques of caries-free patients (p < 0.05). Conclusion: This study is one of the first studies examining the microbial composition of dental plaques with black staining in carious and caries-free adult patients using next generation sequencing technology. In the presence of black staining, plaques have an ultimate complex microbial structure. A lower caries burden was noted in the presence of black staining. WOS:000659789300008 34058724 Q2 |
| Databáze: | OpenAIRE |
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