Suppressed expression of the cytochrome P45017 alpha protein in the testicular feminized (Tfm) mouse testes
| Autor: | C. Le Goascogne, N. Sananès, Paul Robel, Etienne-Emile Baulieu, M. Gouézou |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Cytochrome Ratón Endocrinology Diabetes and Metabolism Biology Testicle Organ culture Mice Endocrinology Organ Culture Techniques Internal medicine Testis medicine Animals Bucladesine Progesterone Reductase Cholesterol side-chain cleavage enzyme Cytochrome P450 Leydig Cells Steroid 17-alpha-Hydroxylase Androgen-Insensitivity Syndrome medicine.disease Immunohistochemistry Mice Mutant Strains medicine.anatomical_structure biology.protein Androgen insensitivity syndrome medicine.drug |
| Zdroj: | The Journal of endocrinology. 139(1) |
| ISSN: | 0022-0795 |
| Popis: | The testes of testicular feminized (Tfm) mice synthesize and secrete abnormally low amounts of testosterone, as a consequence of selectively decreased cytochrome P45017α activity. To investigate the mechanism of this deficiency, three steroidogenic enzymes were immunolabelled in the testes of normal and Tfm adult (2·5–6 month old) mice. Cholesterol side-chain cleavage cytochrome P450 (P450scc) and Δ5-3β-hydroxysteroid dehydrogenase (3β-HSD) were detected in the Leydig cells of both normal and Tfm mice whereas, in contrast to normal mice, only a small proportion of Leydig cells were immunostained for cytochrome P450-17α-hydroxylase,C17→20 lyase (P45017α) in the testes of Tfm mice. The numbers of cells differed from male to male and interestingly were markedly higher in the right testis. Explants of testes from Tfm mice were kept in organ culture at 32 °C for 45 h, with or without dibutyryl cyclic AMP (100 or 500 μmol/l). All Leydig cells remained positive for P450scc and 3β-HSD, and P45017α became detectable in the majority of Leydig cells in both left and right testes, showing that the lack of expression of P45017α protein in Tfm mouse testes in vivo is not structural but is a regulatory phenomenon. Journal of Endocrinology (1993) 139, 127–130 |
| Databáze: | OpenAIRE |
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