Identification of intact glucosinolates using direct coupling of high-performance liquid chromatography with continuous-flow frit fast atom bombardment tandem mass spectrometry

Autor: J. van der Greef, U.R. Tjaden, G. van de Werken, P. S. Kokkonen, Wilfried M. A. Niessen, G. J. ten Hove
Rok vydání: 1991
Předmět:
Zdroj: Scopus-Elsevier
ISSN: 1096-9888
1052-9306
DOI: 10.1002/bms.1200200505
Popis: Intact glucosinolates have been separated and identified in standard mixtures using liquid chromatography (LC) and continuous-flow frit fast atom bombardment tandem mass spectrometry. The separation of individual glucosinolates is achieved by using a conventional-bore LC column with an aqueous ammonium acetate-acetonitrile gradient. The mandatory flow rate reduction is achieved by using a pneumatic splitter. The daughter ion spectra after collision-induced dissociation of the characteristic [M − H]− anion of the glucosinolates yields extensive structure information. Both group- and compound-specific fragments and group-characteristic neutral losses are observed. Strategies for identification of unknown glucosinolates in a mixture from their negative ion tandem mass spectra are indicated.
Databáze: OpenAIRE
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