Chitobiose alleviates oleic acid-induced lipid accumulation by decreasing fatty acid uptake and triglyceride synthesis in HepG2 cells
| Autor: | Liming Zhao, Xiaodan Li, Y. Martin Lo, Liehuan Chen, Liqiang Fan, Mengyao Zhao, Junhui Chen, Xuni Cao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hepatic steatosis CD36 Medicine (miscellaneous) Chitobiose 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine TX341-641 Liver X receptor DGAT2 chemistry.chemical_classification Nutrition and Dietetics Triglyceride biology Nutrition. Foods and food supply Fatty acid Peroxisome medicine.disease Oleic acid 030104 developmental biology Biochemistry chemistry biology.protein 030211 gastroenterology & hepatology lipids (amino acids peptides and proteins) Steatosis Food Science |
| Zdroj: | Journal of Functional Foods, Vol 46, Iss, Pp 202-211 (2018) |
| ISSN: | 1756-4646 |
| Popis: | This study investigates the ameliorative effect of crude chitooligosaccharide (COS) and five specific COSs ((GlcN)2-6) on lipid accumulation and, therein, characterizes the inhibition mechanism of chitobiose ((GlcN)2). After treatment with oleic acid (OA), the triglyceride (TG), LDL-c content, lipogenesis-signaling genes and protein in HepG2 cells increased, while lipid accumulation was suppressed by COS and five single COSs both in co-treatment and after-treatment. In addition, we observed that 4 mg/mL (GlcN)2 had a significant inhibitory effect on hepatic lipid accumulation and decreased the mRNA and protein expressions of diacylglycerol acyltransferase 2 (DGAT2), liver X receptor α (LXRα), peroxisome proliferator-activated receptor-γ (PPARγ), pregnenolone X receptor (PXR) and cluster of differentiation 36 (CD36). These results collectively indicate that, among all (GlcN)2-6 studied, (GlcN)2 provides the best active effect on anti-hyperlipidemia and steatosis regulation via decreasing fatty acid uptake and TG synthesis in HepG2 cells. |
| Databáze: | OpenAIRE |
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