Berberis microphylla G. Forst (Calafate) Berry Extract Reduces Oxidative Stress and Lipid Peroxidation of Human LDL
| Autor: | Andy J. Pérez, Claudia Radojkovic, Si Yen Chau, Daniela Nova, Lia Olivares-Caro, Claudia Mardones, José Y. Neira, Luis Bustamante |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology Linoleic acid Clinical Biochemistry Oxidative phosphorylation phenolic compounds antioxidant capacity Berberis microphylla G. forst medicine.disease_cause 01 natural sciences Biochemistry Article Umbilical vein Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound cardiovascular disease medicine Food science Molecular Biology 030109 nutrition & dietetics biology 010401 analytical chemistry lcsh:RM1-950 Berberis microphylla human umbilical vein endothelial cells (HUVEC) Cell Biology Ultra Liquid Chromatography with diode array detector coupled to Quadrupole-Time of Fly Mass Spectrometry (UHPLC-DAD-QTOF) biology.organism_classification Malondialdehyde 0104 chemical sciences lcsh:Therapeutics. Pharmacology chemistry Gas chromatography–mass spectrometry Oxidative stress |
| Zdroj: | Antioxidants, Vol 9, Iss 1171, p 1171 (2020) Antioxidants Volume 9 Issue 12 |
| ISSN: | 2076-3921 |
| Popis: | Calafate (Berberis microphylla G. Forst) is a Patagonian barberry very rich in phenolic compounds. Our aim was to demonstrate, through in vitro models, that a comprehensive characterized calafate extract has a protective role against oxidative processes associated to cardiovascular disease development. Fifty-three phenolic compounds (17 of them not previously reported in calafate), were tentatively identified by Ultra-Liquid Chromatography with Diode Array Detector, coupled to Quadrupole-Time of Fly Mass Spectrometry (UHPLC-DAD-QTOF). Fatty acids profile and metals content were studied for the first time, by Gas Chromatography Mass Spectrometry (GC-MS) and Total X-ray Fluorescence (TXRF), respectively. Linolenic and linoleic acid, and Cu, Zn, and Mn were the main relevant compounds from these groups. The bioactivity of calafate extract associated to the cardiovascular protection was evaluated using Human Umbilical Vein Endothelial Cells (HUVECs) and human low density lipoproteins (LDL) to measure oxidative stress and lipid peroxidation. The results showed that calafate extract reduced intracellular Reactive Oxygen Species (ROS) production (51%) and completely inhibited LDL oxidation and malondialdehyde (MDA) formation. These findings demonstrated the potential of the relevant mix of compounds found in calafate extract on lipoperoxidation and suggest a promising protective effect for reducing the incidence of cardiovascular disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|