Analysis of glucose and lactate in dialysate from hypothalamus of rats after exhausting swimming using microdialysis
| Autor: | Lei Wang, Huiwan Han, Gangyi Liu, Xingju Yu, Rui Zhao, Yu Dong, Dihua Shangguan |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Microdialysis Clinical Biochemistry Hypothalamus Sensitivity and Specificity Biochemistry Analytical Chemistry Rats Sprague-Dawley Internal medicine Drug Discovery Extracellular fluid Electrochemistry medicine Animals Lactic Acid Molecular Biology Swimming Pharmacology Chromatography Chemistry Reproducibility of Results General Medicine Enzymes Immobilized Rats Glucose Endocrinology Basal (medicine) Flow Injection Analysis human activities Nadir (topography) |
| Zdroj: | Biomedical Chromatography. 16:427-431 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/bmc.177 |
| Popis: | A microbore flow injection analysis-immobilized enzyme reactor-electrochemical detection (FIA-IMER-ECD) system for glucose and lactate detection was built up. The assays were precise, sensitive and practicable for determination of glucose and lactate levels in hypothalamic dialysate. The method had been used to detect the dynamic changes of glucose and lactate levels during rat exhausting swimming and recovery. The data showed that after exhausting swimming, the concentration of glucose in hypothalamic dialysate that reflected the concentration in the hypothalamic extracellular fluid decreased. The level fell to its nadir at day 1 after the exercise and then went back to the basal level at day 3 after the swimming. However, lactate levels increased to a maximum at day 3 and went back to the basal level at day 5 after the swimming. |
| Databáze: | OpenAIRE |
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