Ultrasound-enhanced delivery of antibiotics and anti-inflammatory drugs into the eye
| Autor: | Marjan Nabili, Vesna Zderic, Ji Liu, Craig Geist, Hetal Patel, Sankaranarayana P. Mahesh |
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| Rok vydání: | 2012 |
| Předmět: |
Electrophoresis
medicine.medical_specialty Acoustics and Ultrasonics medicine.drug_class medicine.medical_treatment Biophysics Anti-Inflammatory Agents Administration Ophthalmic In Vitro Techniques Anti-inflammatory Article Cornea Sonication Ophthalmology medicine Tobramycin Animals Radiology Nuclear Medicine and imaging Tissue Distribution Dexamethasone Radiological and Ultrasound Technology Therapeutic ultrasound business.industry Ultrasound Sonophoresis eye diseases Surgery Anti-Bacterial Agents medicine.anatomical_structure Drug delivery sense organs Rabbits Ophthalmic Solutions business medicine.drug |
| Zdroj: | Ultrasound in medicinebiology. 39(4) |
| ISSN: | 1879-291X |
| Popis: | Delivery of sufficient amounts of therapeutic drugs into the eye is often a challenging task. In this study, ultrasound application (frequencies of 400 KHz to 1 MHz, intensities of 0.3–1.0 W/cm2 and exposure duration of 5 min) was investigated to overcome the barrier properties of cornea, which is a typical route for topical administration of ophthalmic drugs. Permeability of ophthalmic drugs, tobramycin and dexamethasone and sodium fluorescein, a drug-mimicking compound, was studied in ultrasound- and sham-treated rabbit corneas in vitro using a standard diffusion cell setup. Light microscopy observations were used to determine ultrasound-induced structural changes in the cornea. For tobramycin, an increase in permeability for ultrasound- and sham-treated corneas was not statistically significant. Increase of 46%–126% and 32%–109% in corneal permeability was observed for sodium fluorescein and dexamethasone, respectively, with statistical significance (p < 0.05) achieved at all treatment parameter combinations (compared with sham treatments) except for 1-MHz ultrasound applications for dexamethasone experiments. This permeability increase was highest at 400 kHz and appeared to be higher at higher intensities applied. Histologic analysis showed structural changes that were limited to epithelial layers of cornea. In summary, ultrasound application provided enhancement of drug delivery, increasing the permeability of the cornea for the anti-inflammatory ocular drug dexamethasone. Future investigations are needed to determine the effectiveness and safety of this application in in vivo long-term survival studies. (E-mail: mnabili@gwu.edu) |
| Databáze: | OpenAIRE |
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