Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors
| Autor: | Aline Bayerle, Hermann Josef Gröne, Florian Guillou, Stefan Porubsky, Hans Heid, Jens Fuchser, Christian Marsching, Roger Sandhoff, Christian Bolenz, Karin Gorgas, Mariona Rabionet, Richard Jennemann |
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| Přispěvatelé: | Lipid Pathobiochemistry Group, Department of Cellular and Molecular Pathology, Department of Cellular and Molecular Pathology, German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (DKFZ), University of Liverpool, Helmholtz Group for Cell Biology, Bruker Daltonik GmbH, Center for Applied Research, Applied Biomedical Mass Spectrometry ( ABIMAS), Department of Pathology, University of Alabama at Birmingham [ Birmingham] (UAB), Department of Urology, University Medical Center Mannheim, University of Heidelberg, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Instrumental Analytics and Bioanalytics, University of Mannheim, German Research Foundation (DFG) SA1721/1-2 SA 1721/2-1 GO 432/2-1, SFB grant 1118, Heidelberg University, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Cell division mass spectrum analysis Gene Expression Apoptosis spermiocytes Spermatocyte Mice spectrométrie de masse Sphingosine N-Acyltransferase Testis meiosis Genetics (clinical) souris knock out spermatogénèse Ceramide synthase 3 Fatty Acids General Medicine Cell biology medicine.anatomical_structure spermatocyte Meiotic cytokinesis méiose [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] cytokinese Biology man Genetics medicine Animals Humans RNA Messenger Spermatogenesis Molecular Biology Mitosis Cytokinesis Sphingolipids apoptose Spermatid Cell Membrane Sphingolipid sphingolipide Germ Cells cellule somatique Infertility biology.protein somatic cell sphingolipid humain spermiation |
| Zdroj: | Human Molecular Genetics Human Molecular Genetics, Oxford University Press (OUP), 2015, 24 (17), pp.4792-4808. ⟨10.1093/hmg/ddv204⟩ |
| ISSN: | 0964-6906 1460-2083 |
| Popis: | Somatic cell cytokinesis was shown to involve the insertion of sphingolipids (SLs) to midbodies prior to abscission. Spermatogenic midbodies transform into stable intercellular bridges (ICBs) connecting clonal daughter cells in a syncytium. This process requires specialized SL structures. (1) Using high resolution-mass spectrometric imaging, we show in situ a biphasic pattern of SL synthesis with testis-specific anchors. This pattern correlates with and depends on ceramide synthase 3 (CerS3) localization in both, pachytene spermatocytes until completion of meiosis and elongating spermatids. (2) Blocking the pathways to germ cell-specific ceramides (CerS3-KO) and further to glycosphingolipids (glucosylceramide synthase-KO) in mice highlights the need for special SLs for spermatid ICB stability. In contrast to somatic mitosis these SLs require ultra-long polyunsaturated anchors with unique physico-chemical properties, which can only be provided by CerS3. Loss of these anchors causes enhanced apoptosis during meiosis, formation of multinuclear giant cells and spermatogenic arrest. Hence, testis-specific SLs, which we also link to CerS3 in human testis, are quintessential for male fertility. |
| Databáze: | OpenAIRE |
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