A chimaeric hygromycin resistance gene as a selectable marker in plant cells

Autor:	John Bedbrook, K. Y. Lee, P. J. M. Van Den Elzen, Jeffrey Townsend
Rok vydání:	1985
Předmět:	Genetics Nicotiana tabacum Genetic transfer Plant Science General Medicine Biology Plant cell biology.organism_classification Molecular biology carbohydrates (lipids) chemistry.chemical_compound Transformation (genetics) Ti plasmid chemistry Agronomy and Crop Science Gene Selectable marker DNA
Zdroj:	Plant molecular biology. 5(5)
ISSN:	0167-4412
Popis:	We show here that plant cells are sensitive to the antibiotic hygromycin-B(4). We also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) gene, when transferred to plants' cells, confers resistance to hygromycin B. The chimaeric nos-hpt gene enables efficient selection of DNA transfer to plant cells when used in conjunction with Ti plasmid-derived binary vectors in cocultivation experiments.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::502e633449991d8973df9917cba8fa2d https://pubmed.ncbi.nlm.nih.gov/24306921 Zobrazit plný text záznamu Full text from SpringerLink