Aerobic Exercise During Advance Stage of Uncontrolled Arterial Hypertension
| Autor: | Antonio Carlos Cicogna, Katashi Okoshi, Ana Lloret, Ricardo Luiz Damatto, Tulio M. M. Caldonazo, Luana Urbano Pagan, Marcelo Diarcadia Mariano Cezar, Dijon Henrique Salomé de Campos, Ana Angélica Henrique Fernandes, Aline R. R. Lima, David Rafael Abreu Reyes, Denise C. Fernandes, Marina Politi Okoshi, Felipe C. Damatto, Francisco R.M. Laurindo, Bertha F. Polegato, Mariana Janini Gomes |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP), FAIT, Universidade de São Paulo (USP), INCLIVA |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
hypertension Physiology Physical exercise heart myocardium function medicine.disease_cause chemistry.chemical_compound physical exercise Physiology (medical) Internal medicine Lactate dehydrogenase Medicine Aerobic exercise Citrate synthase QP1-981 cardiovascular diseases Original Research biology business.industry Malondialdehyde medicine.disease Endocrinology chemistry Heart failure biology.protein cardiovascular system business spontaneously hypertensive rats Nicotinamide adenine dinucleotide phosphate Oxidative stress |
| Zdroj: | Frontiers in Physiology, Vol 12 (2021) Frontiers in Physiology Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-29T08:45:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-03 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Universidade Estadual Paulista Aim: To evaluate the influence of physical training on myocardial function, oxidative stress, energy metabolism, and MAPKs and NF-κB signaling pathways in spontaneously hypertensive rats (SHR), at advanced stage of arterial hypertension, which precedes heart failure development. Methods: We studied four experimental groups: normotensive Wistar rats (W, n = 27), trained W (W-EX, n = 31), SHR (n = 27), and exercised SHR (SHR-EX, n = 32). At 13 months old, the exercise groups underwent treadmill exercise 5 days a week for 4 months. In vitro myocardial function was analyzed in left ventricular (LV) papillary muscle preparations. Antioxidant enzyme activity and energy metabolism were assessed by spectrophotometry. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity was analyzed by lucigenin reduction and protein expression by Western blot. Statistical analyzes: ANOVA and Tukey or Kruskal–Wallis and Dunn tests. Results: SHR-EX had a lower frequency of heart failure features than SHR. Myocardial function and antioxidant enzyme activity were better in SHR-EX than SHR. Lipid hydroperoxide concentration, and phosphorylated JNK and total IkB protein expression were higher in hypertensive than control groups. Malondialdehyde, NADPH oxidase activity, total JNK, phosphorylated p38, phosphorylated and total p65 NF-κB, and phosphorylated IkB did not differ between groups. Protein expression from total p38, and total and phosphorylated ERK were higher in SHR than W. Lactate dehydrogenase and phosphorylated ERK were lower and citrate synthase and β-hydroxyacyldehydrogenase were higher in SHR-EX than SHR. Conclusion: Exercise improves physical capacity, myocardial function, and antioxidant enzyme activity; reduces the frequency of heart failure features and ERK phosphorylation; and normalizes energy metabolism in SHR. Botucatu Medical School UNESP Sao Paulo State University Itapeva Social and Agrarian Sciences College FAIT Department of Cardiopneumology Medical School University of Sao Paulo (USP) Institute of Biosciences Sao Paulo State University (UNESP) Freshage Research Group Department of Physiology University of Valencia CIBERFES INCLIVA Botucatu Medical School UNESP Sao Paulo State University Institute of Biosciences Sao Paulo State University (UNESP) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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