Additional file 2 of Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip

Autor: Mahajan, Gautam, Doherty, Erin, To, Tania, Sutherland, Arlene, Grant, Jennifer, Junaid, Abidemi, Gulati, Aakanksha, LoGrande, Nina, Izadifar, Zohreh, Timilsina, Sanjay Sharma, Horváth, Viktor, Plebani, Roberto, France, Michael, Hood-Pishchany, Indriati, Rakoff-Nahoum, Seth, Kwon, Douglas S., Goyal, Girija, Prantil-Baun, Rachelle, Ravel, Jacques, Ingber, Donald E.
Rok vydání: 2022
DOI: 10.6084/m9.figshare.21628687.v1
Popis: Additional file 2: Supplementary Figure S2. Culture of L. crispatus and non-optimal G. vaginalis containing consortia in the Vagina Chip. A) CFU/Chip for L. crispatus is determined by quantifying non-adherent bacteria in effluents (total of 24, 48- and 72-hours) from the apical epithelial channel and viable bacteria adherent to the epithelium measured within tissue digests at 72-hours. B) CFU/Chip of BVC1 and BVC2 consortia bacteria measured in effluents (total of 24, 48- and 72-hours) from the vaginal epithelium-lined channel and epithelial tissue digest at 72-hours. C) D-lactate Production in Vagina Chips. D-lactate concentrations measured in effluents from the apical epithelial channel of chips cultured in the absence (Control) or presence of the OC1, OC2, or OC3 L. crispatus consortia collected at 24 hours post inoculation are shown; each data point indicates one chip.
Databáze: OpenAIRE