Stability of varicella-zoster virus open reading frame 63
| Autor: | Abbas Vafai, Angela Liu, Nicholas Vafai, John Hart, Ju He, Hsi Liu, Xiaoling Tang, Merry Liu, Dongxia Wang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Herpesvirus 3 Human medicine.drug_class viruses Blotting Western DNA Mutational Analysis Monoclonal antibody medicine.disease_cause Herpesviridae Virus Cell Line Immediate-Early Proteins Gene product Viral Envelope Proteins Virology Alphaherpesvirinae Chlorocebus aethiops medicine Animals Humans Point Mutation Gene biology Varicella zoster virus virus diseases Sequence Analysis DNA General Medicine biology.organism_classification Molecular biology Molecular Weight Open reading frame DNA Viral |
| Zdroj: | Archives of Virology. 153:1943-1947 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-008-0197-4 |
| Popis: | The stability of varicella-zoster virus (VZV) open reading frame (ORF) 63 was analyzed by sequential passage of a virus strain in cell culture. VZV was propagated in culture for 1,206 passages. ORF63 from six passages (18, 220, 516, 730, 1060, and 1,206) was selected and sequenced. Among the six passages, only passage 1,206 showed point mutations at three locations: 551, 618 and 661. In addition, western blot analysis with anti-ORF63 monoclonal antibodies showed no discernable difference in the size of the ORF63 gene product from passage 18 and that from passage 1,206. These results indicate the stability of VZV ORF63 gene in culture over 1,206 passages. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink