Elevation of neuron-specific enolase and S-100beta protein level in experimental acute spinal cord injury
| Autor: | Gu Li, Weiguo Liu, Fang Shen, Fei Cao, Xiaofeng Yang, Xue-qun Zhao, Shi-ting Lv, Weiwei Hu, Xiu-Jue Zheng |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Enolase Enzyme-Linked Immunosorbent Assay S100 Calcium Binding Protein beta Subunit Severity of Illness Index Rats Sprague-Dawley Animal model Cerebrospinal fluid Physiology (medical) medicine Animals Nerve Growth Factors Spinal cord injury Spinal Cord Injuries Neurologic Examination business.industry S100 Proteins General Medicine medicine.disease S 100β protein Rats Disease Models Animal nervous system Neurology Phosphopyruvate Hydratase Acute spinal cord injury Biomarker (medicine) Surgery Neurology (clinical) business |
| Zdroj: | Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia. 15(5) |
| ISSN: | 0967-5868 |
| Popis: | We evaluated the protein levels of neuron-specific enolase (NSE) and S-100beta in serum and cerebrospinal fluid (CSF) in an animal model of acute spinal cord injury and ascertained their relevance. Spinal cord injury was induced at the T8 level in rats. Enzyme-linked immunosorbent assay was used to measure the protein levels of NSE and S-100beta in both serum and CSF at different time points (30 min, 2 h, 6 h, 12 h and 24 h after induction of spinal cord injury). There existed a significant correlation between neurological deficits and the severity of spinal cord injury (p0.05). Compared with the control group, the protein levels of NSE and S-100beta in serum and CSF significantly increased from 2 h after injury (p0.05) and reached a maximum at 6 h. Within a certain time window, the protein levels of NSE and S-100beta in serum and CSF were closely related to the severity of injury level (p0.05). The protein levels of NSE and S-100beta in serum and CSF significantly increased after experimental spinal cord injury in a time-dependent manner and thus may be considered specific biomarkers for acute spinal cord injury. |
| Databáze: | OpenAIRE |
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