Enhancing Hepatocyte Adhesion by Pulsed Plasma Deposition and Polyethylene Glycol Coupling
| Autor: | Richard B. Timmons, Kevin D. Nelson, Paul E. Bankey, Brett E. Thomes, Anca Constantinescu, Muthumarthanda R. Mariappan, E. Carlisle, Robert C. Eberhart |
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| Rok vydání: | 2000 |
| Předmět: |
Polymers
Surface Properties Polyesters Biomedical Engineering Biocompatible Materials Peptide Polyethylene glycol Cell Line Polyethylene Glycols Extracellular matrix Mice chemistry.chemical_compound Materials Testing PEG ratio Cell Adhesion medicine Animals MTT assay Lactic Acid chemistry.chemical_classification technology industry and agriculture General Engineering Adhesion medicine.anatomical_structure Liver chemistry Hepatocyte Polycaprolactone Biophysics Oligopeptides |
| Zdroj: | Tissue Engineering. 6:45-52 |
| ISSN: | 1557-8690 1076-3279 |
| DOI: | 10.1089/107632700320883 |
| Popis: | Decreased hepatocyte adhesion to polymeric constructs limits the function of tissue engineered hepatic assist devices. We grafted adhesion peptides (RGD and YIGSR) to polycaprolactone (PCL) and poly-L-lactic acid (PLLA) in order to mimic the in vivo extracellular matrix and thus enhance hepatocyte adhesion. Peptide grafting was done by a novel technique in which polyethylene glycol (PEG)-adhesion peptide was linked to allyl-amine coated on the surface of PCL and PLLA by pulsed plasma deposition (PPD). Peptide grafting density, quantified by radio-iodinated tyrosine in YIGSR, was 158 fmol/cm(2) on PLLA and 425 fmol/cm(2) on PCL surfaces. The adhesion of hepatocytes was determined by plating 250,000 hepatocytes/well (test substrates were coated on 12 well plates) and quantifying the percentage of adhered cells after 6 h by MTT assay. Adhesion on PCL surfaces was significantly enhanced (p0.05) by both YIGSR (percentage of adhered cells = 53 +/- 7%) and RGD (53 +/- 12%) when compared to control surfaces (31 +/- 8%). Hepatocyte adhesion on PLLA was significantly (p0.05) enhanced on PLLA-PEG-RGD surfaces (76 +/- 14%) compared to control surfaces (42 +/- 19%) and more (68 +/- 25%) but not statistically significant (p = 0.15) on PLLA-PEG-YIGSR surfaces compared to control surfaces. These results indicate that hepatocyte adhesion to PCL and PLLA based polymeric surfaces can be enhanced by a novel adhesion peptide grafting technique using pulsed plasma deposition and PEG cross-linking. |
| Databáze: | OpenAIRE |
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