Stiffness of photocrosslinked RGD-alginate gels regulates adipose progenitor cell behavior
| Autor: | Emily M. Chandler, Brian Kirby, Jason S. Lee, William J. Polacheck, Caroline M. Berglund, Jason P. Gleghorn, Claudia Fischbach |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Light
Tissue Engineering Alginates Chemistry Hexuronic Acids Stem Cells Regeneration (biology) Adipose tissue Bioengineering Nanotechnology Context (language use) Matrix (biology) Applied Microbiology and Biotechnology Hydrogel Polyethylene Glycol Dimethacrylate Cell biology Extracellular matrix Adipose Tissue Glucuronic Acid Adipogenesis Self-healing hydrogels Progenitor cell Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 108:1683-1692 |
| ISSN: | 0006-3592 |
| DOI: | 10.1002/bit.23079 |
| Popis: | Adipose progenitor cells (APCs) are widely investigated for soft tissue reconstruction following tumor resection; however, the long-term success of current approaches is still limited. In order to develop clinically relevant therapies, a better understanding of the role of cell-microenvironment interactions in adipose tissue regeneration is essential. In particular, the effect of extracellular matrix (ECM) mechanics on the regenerative capability of APCs remains to be clarified. We have used artificial ECMs based on photocrosslinkable RGD-alginate to investigate the adipogenic and pro-angiogenic potential of 3T3-L1 preadipocytes as a function of matrix stiffness. These hydrogels allowed us to decouple matrix stiffness from changes in adhesion peptide density or extracellular Ca(2+) concentration and provided a physiologically relevant 3D culture context. Our findings suggest that increased matrix rigidity promotes APC self-renewal and angiogenic capacity, whereas, it inhibits adipose differentiation. Collectively, this study advances our understanding of the role of ECM mechanics in adipose tissue formation and vascularization and will aid in the design of efficacious biomaterial scaffolds for adipose tissue engineering applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text