Specific bioanalytical optical and photoelectrochemical assays for detection of methanol in alcoholic beverages
| Autor: | Marco Möller, Nerea Briz, Laura Saa, Beatriz Díez-Buitrago, Javier Barroso, Valeri Pavlov |
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| Rok vydání: | 2018 |
| Předmět: |
Photoelectrochemistry
Biomedical Engineering Biophysics Biosensing Techniques 02 engineering and technology Sulfides 01 natural sciences Fluorescence Pichia Fluorescence spectroscopy chemistry.chemical_compound Limit of Detection Quantum Dots Cadmium Compounds Electrochemistry Organic chemistry Cysteine Hydrogen peroxide Detection limit Photocurrent Ethanol Alcoholic Beverages Methanol 010401 analytical chemistry technology industry and agriculture Electrochemical Techniques Hydrogen Peroxide General Medicine Enzymes Immobilized 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences Alcohol oxidase Alcohol Oxidoreductases chemistry 0210 nano-technology Oxidation-Reduction Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 101:116-122 |
| ISSN: | 0956-5663 |
| Popis: | Methanol is a poison which is frequently discovered in alcoholic beverages. Innovative methods to detect methanol in alcoholic beverages are being constantly developed. We report for the first time a new strategy for the detection of methanol using fluorescence spectroscopy and photoelectrochemical (PEC) analysis. The analytical system is based on the oxidation of cysteine (CSH) with hydrogen peroxide (H2O2) enzymatically generated by alcohol oxidase (AOx). H2O2 oxidizes capping agent CSH, modulating the growth of CSH-stabilized cadmium sulphide quantum dots (CdS QDs). Disposable screen-printed carbon electrodes (SPCEs) modified with a conductive osmium polymer (Os-PVP) complex were employed to quantify resulting CdS QDs. This polymer facilitates the “wiring” of in situ enzymatically generated CdS QDs, which photocatalyze oxidation of 1-thioglycerol (TG), generating photocurrent as the readout signal. Likewise, we proved that our systems did not suffer from interference by ethanol. The PEC assays showed better sensitivity than conventional methods, covering a wide range of potential applications for methanol quantification. the Spanish Ministry of Economy and Competitiveness (project BIO2014-59741). |
| Databáze: | OpenAIRE |
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