Salivary Epstein-Barr virus DNA level in patients with nasopharyngeal carcinoma following radiotherapy
| Autor: | Mike Y.T. Law, Edmond H.N. Pow, Ranawaka A.P.M. Perera, Peter C.S. Tsang, Dora L.W. Kwong |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Cancer Research Saliva Herpesvirus 4 Human Adolescent Biology medicine.disease_cause Polymerase Chain Reaction Virus law.invention Pathogenesis chemistry.chemical_compound Young Adult law hemic and lymphatic diseases medicine Humans Polymerase chain reaction Aged Nasopharyngeal Neoplasms Middle Aged medicine.disease Virology Epstein–Barr virus Real-time polymerase chain reaction Oncology Nasopharyngeal carcinoma chemistry Immunology DNA Viral Hong Kong Female Oral Surgery DNA |
| Zdroj: | Oral oncology. 47(9) |
| ISSN: | 1879-0593 |
| Popis: | Nasopharyngeal carcinoma (NPC) is a solid tumor closely associated with Epstein-Barr virus (EBV) infection. The purpose of this investigation was to detect and quantify the EBV DNA level in salivary samples of NPC patients following treatment using real-time PCR. A total of 175 consecutive newly diagnosed NPC patients' whole saliva samples were collected before treatment, and the EBV DNA level was measured by real-time PCR, with the primers and probe targeting the BamHI-W region of the EBV genome. The post-treatment salivary EBV DNA level was also assessed in 46 patients. The change of EBV DNA level before and after treatment and relationship of EBV DNA level to demographic data and tumor staging were tested by Wilcoxon signed-rank test and Mann-Whitney U test, respectively with the level of significance set at 0.05. The EBV detection rate of pre-treatment saliva samples was 80%. The EBV DNA level of post-treatment saliva samples was significantly higher than the pre-treatment ones (P |
| Databáze: | OpenAIRE |
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