Successful control of a neonatal outbreak caused mainly by ST20 multidrug-resistant SHV-5-producing Klebsiella pneumoniae, Greece
| Autor: | Antonios Gounaris, Vivi Miriagou, Apostolos Liakopoulos, Angeliki Mavroidi, Katerina Gaitana, Maria Goudesidou, Efthymia Petinaki |
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| Rok vydání: | 2014 |
| Předmět: |
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Bacterial Male NICU Catheterization Central Venous Parenteral Nutrition Pediatrics medicine.medical_specialty Neonatal intensive care unit Klebsiella pneumoniae medicine.medical_treatment Gestational Age Molecular typing beta-Lactamases Disease Outbreaks Risk Factors Drug Resistance Multiple Bacterial Intensive Care Units Neonatal medicine Humans Infection control Pediatrics Perinatology and Child Health Cross Infection Infection Control Greece biology Cesarean Section business.industry Infant Newborn Gestational age Outbreak Infant Low Birth Weight biology.organism_classification Klebsiella Infections Multiple drug resistance ESBL Chest Tubes Pediatrics Perinatology and Child Health Beta-lactamase Multilocus sequence typing Female Intubation business Research Article |
| Zdroj: | BMC Pediatrics BMC Pediatrics, 14, 105 |
| ISSN: | 1471-2431 |
| DOI: | 10.1186/1471-2431-14-105 |
| Popis: | Background Extended spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-Kp) infection can cause significant morbidity and mortality in neonates. We investigated a nosocomial ESBL-Kp outbreak in a neonatal intensive care unit (NICU) of the University Hospital of Larissa (UHL), Central Greece. Methods A total of sixty-four ESBL-Kp were studied; twenty six isolates were recovered from the NICU and were compared with thirty-eight randomly selected isolates from different wards of the hospital during the period March- December 2012. All isolates were characterized by antimicrobial susceptibility testing, ESBL-production by double-disk synergy test, molecular typing using BOX-PCR, whereas selected isolates were further characterized by beta lactamase and virulence gene content, multilocus sequence typing and phylogenetic analysis. All neonates affected by ESBL-Kp were put under strict contact isolation, along with appropriate infection control measures. Results The outbreak strain of ST20 multidrug-resistant SHV-5-producing K. pneumoniae was identified in all infected (n = 13) and three colonized neonates. A novel ST (ST1114) was also identified among SHV-5 producers (n = 10) recovered from nine colonized infants, but it was not related with ST20. Both STs were identified only in the NICU and not in other wards of the hospital. No ESBL-Kp were isolated from the hands of the nursing staff and the environment. Although we were not able to identify the source of the outbreak, no ESBL-Kp were isolated in the NICU after this period and we assumed that the outbreak was successfully controlled. All neonates received parenteral nutrition and most of them were delivered by caesarean section and showed low gestational age ( |
| Databáze: | OpenAIRE |
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