Mechanistic Understanding of the Interactions of Cationic Conjugated Oligo- and Polyelectrolytes with Wild-type and Ampicillin-resistant Escherichia coli
| Autor: | Rajib Saha, Anandakumar Sarella, Shyambo Chatterjee, Taity Changa, Ehsan Zamani, Cheryl M. Immethun, Shudipto K. Dishari |
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| Rok vydání: | 2019 |
| Předmět: |
lcsh:Medicine
02 engineering and technology Conjugated polymers Bacterial growth 010402 general chemistry medicine.disease_cause 01 natural sciences Article Cell membrane Amp resistance Cations Escherichia coli medicine lcsh:Science Lipid bilayer Nanoscale materials Multidisciplinary biology Chemistry Soft materials lcsh:R Cell Membrane 021001 nanoscience & nanotechnology biology.organism_classification Polyelectrolyte Anti-Bacterial Agents 0104 chemical sciences medicine.anatomical_structure Biophysics lcsh:Q Ampicillin Cell envelope 0210 nano-technology Ampicillin Resistance Bacteria |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| Popis: | An in-depth understanding of cell-drug binding modes and action mechanisms can potentially guide the future design of novel drugs and antimicrobial materials and help to combat antibiotic resistance. Light-harvesting π-conjugated molecules have been demonstrated for their antimicrobial effects, but their impact on bacterial outer cell envelope needs to be studied in detail. Here, we synthesized poly(phenylene) based model cationic conjugated oligo- (2QA-CCOE, 4QA-CCOE) and polyelectrolytes (CCPE), and systematically explored their interactions with the outer cell membrane of wild-type and ampicillin (amp)-resistant Gram-negative bacteria, Escherichia coli (E. coli). Incubation of the E. coli cells in CCOE/CCPE solution inhibited the subsequent bacterial growth in LB media. About 99% growth inhibition was achieved if amp-resistant E. coli was treated for ~3–5 min, 1 h and 6 h with 100 μM of CCPE, 4QA-CCOE, and 2QA-CCOE solutions, respectively. Interestingly, these CCPE and CCOEs inhibited the growth of both wild-type and amp-resistant E. coli to a similar extent. A large surface charge reversal of bacteria upon treatment with CCPE suggested the formation of a coating of CCPE on the outer surface of bacteria; while a low reversal of bacterial surface charge suggested intercalation of CCOEs within the lipid bilayer of bacteria. |
| Databáze: | OpenAIRE |
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