INHIBITORY EFFECT ON TUMOUR COLONY FORMATION OF MOUSE SERUM ASSOCIATED WITH TUMOUR RESISTANCE IN VIVO IN SEMI SYNGENEIC MICE
| Autor: | Tore Godal, Stein Gundersen, Irena Grzenlak-Puczyinska, Einar Wibe, S. Funderud |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Lung Neoplasms Lymphoma Mice Inbred Strains Biology Inhibitory postsynaptic potential Cell Line Mice Species Specificity In vivo Colony assay medicine Animals Melanoma Inhibitory effect Lewis lung carcinoma Neoplasms Experimental General Medicine Blood Physiological Phenomena Molecular biology Growth Inhibitors Immunity Innate In vitro Clone Cells Mice Inbred C57BL Colony formation Cell culture Hybridization Genetic |
| Zdroj: | Acta Pathologica Microbiologica Scandinavica Section C Immunology. :223-228 |
| ISSN: | 0304-1328 |
| DOI: | 10.1111/j.1699-0463.1981.tb02691.x |
| Popis: | Differences in tumour susceptibility between strains of mice (C57Bl/6 and C57Bl/6 X DBA/2 = B6D2F1) could be demonstrated for several tumours of C57Bl origin, both solid tumours (B16 melanoma and Lewis lung carcinoma) and lymphomas (RBL-5, 136-3 and ALC). Serum from mice with high tumour resistance in vivo (B6D2F1) showed an inhibitory effect on tumour colony formation in a soft agar colony assay. Serum from mice with lower tumour resistance (C57Bl/6) had no effect. When other F1 hybrids of C57Bl/6 parental origin were tested, the same correlation between in vitro inhibition of tumour colony formation and in vivo susceptibility was found. The serum factor was species non-specific, since the activity was expressed against in vitro grown cell lines of human origin. The tumour colony inhibitory activity was heat sensitive (56 degrees C for 30 minutes), precipitable by (NH4)2SO4, and not removed by adsorption on tumour cells. These results demonstrate the existance of a naturally-occurring humoral tumerostatic factor(s) which correlates to in vivo susceptibility to tumour cells. Its relationship to NK cell activity is discussed. |
| Databáze: | OpenAIRE |
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