Comparison of reprogramming genes in induced pluripotent stem cells and nuclear transfer cloned embryos
Autor: | Tong Li, Qiaoshi Zhao, Guang-Yu Bai, Lian Duan, Ruizhen Sun, Yanli Gu, Jingling Shen, Zhiyan Shan, Zhen-Dong Wang, Lianhong Jin, Rui Yuan, Xing-Hui Shen, Yanshuang Wu, Lei Lei |
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Rok vydání: | 2014 |
Předmět: |
Male
Cancer Research Nuclear Transfer Techniques Somatic cell Induced Pluripotent Stem Cells Biology Real-Time Polymerase Chain Reaction Regenerative Medicine Mice Animals Gene Regulatory Networks RNA Messenger Induced pluripotent stem cell Cells Cultured Embryonic Stem Cells Genetics Regulation of gene expression Reverse Transcriptase Polymerase Chain Reaction Cell Biology Cellular Reprogramming Embryonic stem cell Cell biology Clone Cells Gene expression profiling Mice Inbred C57BL Gene Expression Regulation Somatic cell nuclear transfer Female Stem cell Reprogramming |
Zdroj: | Stem cell reviews and reports. 10(4) |
ISSN: | 2629-3277 |
Popis: | The most effective reprogramming methods, somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs), are widely used in biological research and regenerative medicine, yet the mechanism that reprograms somatic cells to totipotency remains unclear and thus reprogramming efficiency is still low. Microarray technology has been employed in analyzing the transcriptomes changes during iPS reprogramming. Unfortunately, it is difficult to obtain enough DNA from SCNT reconstructed embryos to take advantage of this technology. In this study, we aimed to identify critical genes from the transcriptional profile for iPS reprogramming and compared expression levels of these genes in SCNT reprogramming. By integrating gene expression information from microarray databases and published studies comparing somatic cells with either miPSCs or mouse embryonic stem cells (ESCs), we obtained two lists of co-upregulated genes. The gene ontology (GO) enriched analysis of these two lists demonstrated that the reprogramming process is associated with numerous biological processes. Specifically, we selected 32 genes related to heterochromatin, embryonic development, and cell cycle from our co-upregulated gene datasets and examined the gene expression level in iPSCs and SCNT embryos by qPCR. The results revealed that some reprogramming related genes in iPSCs were also expressed in SCNT reprogramming. We established the network of gene interactions that occur with genes differentially expressed in iPS and SCNT reprogramming and then performed GO analysis on the genes in the network. The network genes function in chromatin organization, heterochromatin, transcriptional regulation, and cell cycle. Further researches to improve reprogramming efficiency, especially in SCNT, will focus on functional studies of these selected genes. |
Databáze: | OpenAIRE |
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