Neutrophil Extracellular Traps are associated with disease severity and microbiota diversity in Chronic Obstructive Pulmonary Disease
| Autor: | J. Stuart Elborn, Guillermo Suarez-Cuartin, Andrew Cassidy, Gisli G. Einarsson, Alison J. Dicker, Sara E. Marshall, Gill Brady, Oriol Sibila, Colin N. A. Palmer, Eleanor G. Pumphrey, Elizabeth Furrie, Megan Crichton, Stuart Schembri, Wasyla Ibrahim, James D. Chalmers, Christopher J. Fong |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Neutrophils Disease GOLD Global Initiative for Obstructive Lung Disease Extracellular Traps Severity of Illness Index Pulmonary Disease Chronic Obstructive 0302 clinical medicine exacerbations Immunology and Allergy OTU Operational taxonomic unit Aged 80 and over COPD EN-RAGE Ligand for the receptor for advanced glycation end products ICS Inhaled corticosteroid biology CAT COPD Assessment Test Microbiota phagocytosis Middle Aged Pathophysiology Obstructive lung disease 3. Good health Myeloperoxidase SWDI Shannon-Wiener species diversity index medicine.symptom MPO Myeloperoxidase Immunology COPD Chronic obstructive pulmonary disease Article chronic obstructive pulmonary disease 03 medical and health sciences medicine Haemophilus species Journal Article Humans PMA Phorbol 12-myristate 13-acetate Microbiome Aged disease business.industry Sputum cfDNA Cell-free DNA Neutrophil extracellular traps chronic obstructive pulmonary MRC Medical Research Council medicine.disease NET Neutrophil extracellular trap respiratory tract diseases 030104 developmental biology 030228 respiratory system biology.protein business |
| Zdroj: | Dicker, A J, Crichton, M L, Pumphrey, E G, Cassidy, A J, Suarez-Cuartin, G, Sibila, O, Furrie, E, Fong, C J, Ibrahim, W, Brady, G, Einarsson, G G, Elborn, J S, Schembri, S, Marshall, S E, Palmer, C NA & Chalmers, J D 2017, ' Neutrophil Extracellular Traps are associated with disease severity and microbiota diversity in Chronic Obstructive Pulmonary Disease ', The Journal of allergy and clinical immunology . https://doi.org/10.1016/j.jaci.2017.04.022 JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname The Journal of Allergy and Clinical Immunology |
| ISSN: | 0091-6749 |
| DOI: | 10.1016/j.jaci.2017.04.022 |
| Popis: | Background Neutrophil extracellular traps (NETs) have been observed in the airway in patients with chronic obstructive pulmonary disease (COPD), but their clinical and pathophysiologic implications have not been defined. Objective We sought to determine whether NETs are associated with disease severity in patients with COPD and how they are associated with microbiota composition and airway neutrophil function. Methods NET protein complexes (DNA-elastase and histone-elastase complexes), cell-free DNA, and neutrophil biomarkers were quantified in soluble sputum and serum from patients with COPD during periods of disease stability and during exacerbations and compared with clinical measures of disease severity and the sputum microbiome. Peripheral blood and airway neutrophil function were evaluated by means of flow cytometry ex vivo and experimentally after stimulation of NET formation. Results Sputum NET complexes were associated with the severity of COPD evaluated by using the composite Global Initiative for Obstructive Lung Disease scale (P Graphical abstract |
| Databáze: | OpenAIRE |
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