Comparison of LightCycler-Based PCR, COBAS Amplicor CMV Monitor, and pp65 Antigenemia Assays for Quantitative Measurement of Cytomegalovirus Viral Load in Peripheral Blood Specimens from Patients after Solid Organ Transplantation
| Autor: | Jutta K. Preiksaitis, Barbara LeBlanc, Xiao-Li Pang, Linda Chui, Jayne Fenton |
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| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Coefficient of variation Congenital cytomegalovirus infection Cytomegalovirus medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Herpesviridae Virus law.invention Viral Matrix Proteins law Betaherpesvirinae Virology medicine Humans Polymerase chain reaction biology virus diseases Organ Transplantation Viral Load Phosphoproteins biology.organism_classification medicine.disease Transplantation Cytomegalovirus Infections DNA Viral Regression Analysis Reagent Kits Diagnostic Viral load |
| Zdroj: | Journal of Clinical Microbiology. 41:3167-3174 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.41.7.3167-3174.2003 |
| Popis: | In order to evaluate the LightCycler-based PCR (LC-PCR) as a diagnostic assay technique, a classical pp65 antigenemia assay and the commercially available COBAS Amplicor CMV Monitor (CACM) assay were compared to the LC-PCR assay for the detection and quantitation of cytomegalovirus (CMV) load in 404 parallel specimens of peripheral blood from 66 patients after solid organ transplantation. A good correlation existed among these three assays ( r ≅ 0.6, P < 0.0001). The LC-PCR assay was the most sensitive (54% of specimens positive) compared to the CACM (48.6%) and the pp65 antigenemia (26%) assays. The LC-PCR assay detected all samples found positive by using both the CMV pp65 antigenemia assay and the CACM assay. The LC-PCR also had the widest dynamic range (from 250 to 10 7 DNA copies/ml of plasma). No cross-reactions were found among CMV and Epstein-Barr virus, varicella-zoster virus, or herpes simplex virus in the LC-PCR by using amplification with specifically designed primer pairs. Precision, expressed as the coefficient of variation, was |
| Databáze: | OpenAIRE |
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