Phosphatidyl-inositide signalling proteins in a novel class of sensory cells in the mammalian olfactory epithelium

Autor:	Jacques Paysan, Roberto Tirindelli, Rebecca Elsaesser, Giorgia Montani
Rok vydání:	2005
Předmět:	Olfactory system Green Fluorescent Proteins Phospholipase C beta Receptors Cytoplasmic and Nuclear Mice Transgenic Sensory system Olfaction Phosphatidylinositols Polymerase Chain Reaction Adenylyl cyclase Mice chemistry.chemical_compound Olfactory Mucosa Genes Reporter TRPC6 Cation Channel medicine Animals Inositol 1 4 5-Trisphosphate Receptors Neurons Afferent DNA Primers TRPC Cation Channels Base Sequence Microvilli biology General Neuroscience Epithelial Cells Immunohistochemistry Olfactory Bulb Cell biology Isoenzymes medicine.anatomical_structure chemistry Type C Phospholipases biology.protein Calcium Channels Olfactory ensheathing glia Stem cell Olfactory marker protein Olfactory epithelium Signal Transduction
Zdroj:	European Journal of Neuroscience. 21:2692-2700
ISSN:	1460-9568 0953-816X
Popis:	Ciliated sensory neurons, supporting cells and basal stem cells represent major cellular components of the main olfactory epithelium in mammals. Here we describe a novel class of sensory cells in the olfactory neuroepithelium. The cells express phospholipase C beta-2 (PLC beta2), transient receptor potential channels 6 (TRPC6) and inositol 3, 4, 5-trisphosphate receptors type III (InsP3R-III). Unlike ciliated olfactory neurons, they express neither olfactory marker protein nor centrin, adenylyl cyclase or cyclic nucleotide-gated cation channels. Typical components of the cytoskeleton of microvilli, ezrin and actin are found co-localized with PLC beta2 and TRPC6 in apical protrusions of the cells. In Ca2+-imaging experiments, the cells responded to odours. They express neuronal marker proteins and possess an axon-like process, but following bulbectomy the cells do not degenerate. Our results suggest a novel class of microvillous secondary chemosensory cells in the mammalian olfactory system. These cells, which utilize phosphatidyl-inositides in signal transduction, represent about 5% of all olfactory cells. Their abundance indicates that they play an important role in stimulus-dependent functions and/or the regeneration of the olfactory system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5015f0cb97a4e93b24e5c452696ead07 https://doi.org/10.1111/j.1460-9568.2005.04108.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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