Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation
| Autor: | Mariana Carriquiry, Andrés Trostchansky, M. Garcia-Roche, Mauricio Mastrogiovanni, Celia Quijano, A. Casal, Mateo Ceriani, D. A. Mattiauda, A. Jasinsky, Adriana Cassina |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Biopsy Mitochondria Liver Mitochondrion medicine.disease_cause Biochemistry 0302 clinical medicine Endocrinology Reproductive Physiology Lactation Grazing Medicine and Health Sciences Medicago Post-Translational Modification Energy-Producing Organelles Flowering Plants Multidisciplinary biology Liver Diseases Respiration Fatty liver Chemical Reactions Eukaryota food and beverages Fabaceae Acetylation Plants Legumes Mitochondria Chemistry medicine.anatomical_structure 030220 oncology & carcinogenesis Physical Sciences Medicine Female Cellular Structures and Organelles Research Article Science Surgical and Invasive Medical Procedures Total mixed ration Gastroenterology and Hepatology Bioenergetics 03 medical and health sciences Animal science Oxygen Consumption Fodder medicine Animals Nutrition Endocrine Physiology Lysine Organisms Biology and Life Sciences Proteins Cell Biology Feeding Behavior biology.organism_classification medicine.disease Diet Fatty Liver Oxidative Stress 030104 developmental biology Cattle Physiological Processes Energy Metabolism Festuca arundinacea Oxidative stress |
| Zdroj: | PLoS ONE, Vol 14, Iss 3, p e0213780 (2019) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Early lactation is an energy-demanding period for dairy cows which may lead to negative energy balance, threatening animal health and consequently productivity. Herein we studied hepatic mitochondrial function in Holstein-Friesian multiparous dairy cows during lactation, under two different feeding strategies. During the first 180 days postpartum the cows were fed a total mixed ration (70% forage: 30% concentrate) ad libitum (non-grazing group, G0) or grazed Festuca arundinacea or Mendicago sativa plus supplementation (grazing group, G1). From 180 to 250 days postpartum, all cows grazed Festuca arundinacea and were supplemented with total mixed ration. Mitochondrial function was assessed measuring oxygen consumption rate in liver biopsies and revealed that maximum respiratory rate decreased significantly in grazing cows during early lactation, yet was unchanged in non-grazing cows during the lactation curve. While no differences could be found in mitochondrial content or oxidative stress markers, a significant increase in protein lysine acetylation was found in grazing cows during early lactation but not in cows from the non-grazing group. Mitochondrial acetylation positively correlated with liver triglycerides and β-hydroxybutyrate plasma levels, well-known markers of negative energy balance, while a negative correlation was found with the maximum respiratory rate and sirtuin 3 levels. To our knowledge this is the first report of mitochondrial function in liver biopsies of dairy cows during lactation. On the whole our results indicate that mitochondrial function is impaired during early lactation in grazing cows and that acetylation may account for changes in mitochondrial function in this period. Additionally, our results suggest that feeding total mixed ration during early lactation may be an efficient protective strategy. |
| Databáze: | OpenAIRE |
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