The zymogen-enteropeptidase system: A practical approach to study the regulation of enzyme activity by proteolytic cleavage
| Autor: | Andrea Cristina Frizzas de Lima, Maria Célia Portella, Karina de Souza Routman, João Martins Pizauro, Jesus Aparecido Ferro |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2004 |
| Předmět: |
Enteropeptidase
chemistry.chemical_classification enteropeptidase biology Chemistry Trypsinogen Proteolytic enzymes proteolytic cleavage Chymotrypsinogen Cleavage (embryo) Trypsin Biochemistry trypsin chemistry.chemical_compound Enzyme chymotrypsin Zymogen medicine biology.protein enzyme regulation Molecular Biology zymogen medicine.drug |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1539-3429 1470-8175 |
| DOI: | 10.1002/bmb.2004.494032010311 |
| Popis: | Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:17:35Z No. of bitstreams: 1 WOS000188380200011.pdf: 109683 bytes, checksum: cf989b47a320d6ab3ba90f3ca7ce40f0 (MD5) Made available in DSpace on 2014-02-26T17:17:35Z (GMT). No. of bitstreams: 1 WOS000188380200011.pdf: 109683 bytes, checksum: cf989b47a320d6ab3ba90f3ca7ce40f0 (MD5) Previous issue date: 2004-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:17:29Z No. of bitstreams: 1 WOS000188380200011.pdf: 109683 bytes, checksum: cf989b47a320d6ab3ba90f3ca7ce40f0 (MD5) Made available in DSpace on 2014-05-20T13:17:29Z (GMT). No. of bitstreams: 1 WOS000188380200011.pdf: 109683 bytes, checksum: cf989b47a320d6ab3ba90f3ca7ce40f0 (MD5) Previous issue date: 2004-01-01 The present research describes an efficient procedure to obtain high levels of trypsinogen and chymotrypsinogen by using a simple, rapid, and easily reproducible method. The extraction process and the time-course of activation of zymogens can be carried out in a single laboratory period, without sophisticated equipment. The main objective was to prepare a laboratory class that would stimulate student interest in enzyme regulation, exploring the fact that the catalytic activity of some enzymes is regulated by different mechanisms. The regulation of proteolytic enzymes requires the synthesis of an inactive zymogen and its being irreversibly switched on by specific proteolytic cleavage. UNESP, Fac Ciências Agr & Vet, Dept Tecnol, BR-14884900 Jaboticabal, SP, Brazil UNESP, Fac Ciências Agr & Vet, Dept Tecnol, BR-14884900 Jaboticabal, SP, Brazil |
| Databáze: | OpenAIRE |
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