Label-Free Magnetic-Field-Assisted Assembly of Layer-on-Layer Cellular Structures
| Autor: | Rakesh P. Sahu, Tamaghna Gupta, Fei Geng, Srivatsa Aithal, Sarah Mishriki, Ishwar K. Puri |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Tissue Engineering 0206 medical engineering Biomedical Engineering Hydrogels 3d model 02 engineering and technology 021001 nanoscience & nanotechnology 020601 biomedical engineering Cell assembly Magnetic field Biomaterials Paramagnetism Magnetic Fields Tissue engineering In vivo Biophysics 0210 nano-technology Layer (electronics) Cell Aggregation Label free |
| Zdroj: | ACS Biomaterials Science & Engineering. 6:4294-4303 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.0c00233 |
| Popis: | Controlled cell assembly is essential for fabricating in vitro 3D models that mimic the physiology of in vivo cellular architectures. Whereas tissue engineering techniques often rely on intrusive magnetic nanoparticles placed in cells and hydrogel encapsulation of cells to produce multilayered cellular constructs, we describe a high-throughput, label-free, and scaffold-free magnetic field-guided technique that assembles cells into a layered aggregate. An inhomogeneous magnetic field influences the diamagnetic cells suspended in a paramagnetic culture medium. Driven by the magnetic susceptibility difference and the field gradient, the cells are displaced toward the region of lowest field strength. Two cell lines are used to demonstrate the sequential assembly of layer-on-layer aggregates in microwells within 6 h. The effect of magnet size on the assembly dynamics is characterized and a microwell size criterion for the highest cell aggregation provided. Label-free magnetic-field-assisted assembly is relevant for on-demand scalable biofabrication of complex layered structures. Potential applications include drug discovery, developmental biology, lab-on-chip devices, and cancer research. |
| Databáze: | OpenAIRE |
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