Pleiotropic effects of alpha-SNAP M105I mutation on oocyte biology: ultrastructural and cellular changes that adversely affect female fertility in mice
| Autor: | María Paz Miró, Maria Matilde de Paola, Marcela Alejandra Michaut, Marcelo H Ratto, Luis Federico Bátiz |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Cell biology Mutation Missense lcsh:Medicine Mice Transgenic Perivitelline space Biology medicine.disease_cause Article Exocytosis purl.org/becyt/ford/1 [https] Mice Medical research Methionine Oogenesis Human fertilization FEMALE FERTILITY medicine Animals Isoleucine lcsh:Science purl.org/becyt/ford/1.6 [https] Metaphase Mutation Multidisciplinary Cortical granule exocytosis SNAPs HYH MICE lcsh:R Homozygote Wild type Oocyte Polyspermy Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Fertility medicine.anatomical_structure Amino Acid Substitution M105I MUTATION Fertilization Oocytes lcsh:Q Female Infertility Female |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019) |
| Popis: | After sperm-oocyte fusion, cortical granules (CGs) located in oocyte cortex undergo exocytosis and their content is released into the perivitelline space to avoid polyspermy. Thus, cortical granule exocytosis (CGE) is a key process for fertilization success. We have demonstrated that alpha-SNAP -and its functional partner NSF- mediate fusion of CGs with the plasma membrane in mouse oocytes. Here, we examined at cellular and ultrastructural level oocytes from hyh (hydrocephalus with hop gait) mice, which present a missense mutation in the Napa gene that results in the substitution of methionine for isoleucine at position 105 (M105I) of alpha-SNAP. Mutated alpha-SNAP was mislocalized in hyh oocytes while NSF expression increased during oocyte maturation. Staining of CGs showed that 9.8% of hyh oocytes had abnormal localization of CGs and oval shape. Functional tests showed that CGE was impaired in hyh oocytes. Interestingly, in vitro fertilization assays showed a decreased fertilization rate for hyh oocytes. Furthermore, fertilized hyh oocytes presented an increased polyspermy rate compared to wild type ones. At ultrastructural level, hyh oocytes showed small mitochondria and a striking accumulation and secretion of degradative structures. Our findings demonstrate the negative effects of alpha-SNAP M105 mutation on oocyte biology and further confirm the relevance of alpha-SNAP in female fertility. Fil: de Paola, Maria Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Miró, María Paz. Universidad Austral de Chile; Chile Fil: Ratto, Marcelo Hector. Universidad Austral de Chile; Chile Fil: Bátiz, Luis Federico. Universidad Austral de Chile; Chile. Universidad de Los Andes; Chile Fil: Michaut, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina |
| Databáze: | OpenAIRE |
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