Assessment of a New GC-MS/MS System for the Confirmatory Measurement of PCDD/Fs and (N)DL-PCBs in Food under EU Regulation
Autor: | G. Scholl, Eliane Lazzari, Flavio A. Franchina, Jean-François Focant |
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Rok vydání: | 2019 |
Předmět: |
Spectrum analyzer
Health (social science) Relative standard deviation 02 engineering and technology Plant Science lcsh:Chemical technology Mass spectrometry 01 natural sciences Health Professions (miscellaneous) Microbiology Article NO targeted analysis lcsh:TP1-1185 PE4_5 gas chromatography (GC) PE4_7 Reproducibility Chromatography LS9_6 Chemistry tandem mass spectrometry (MS/MS) 010401 analytical chemistry Selected reaction monitoring method validation Food contaminants Targeted analysis Food safety Gas chromatography (GC) Method validation Tandem mass spectrometry (MS/MS) 021001 nanoscience & nanotechnology 0104 chemical sciences Triple quadrupole mass spectrometer food safety Ionization chamber food contaminants Gas chromatography–mass spectrometry 0210 nano-technology Food Science |
Zdroj: | Foods Volume 8 Issue 8 Foods, Vol 8, Iss 8, p 302 (2019) |
ISSN: | 2304-8158 |
Popis: | Polychlorodibenzo-p-dioxins (PCDDs), polychloro-dibenzofurans (PCDFs), dioxin-like (DL), and non dioxin-like (NDL) polychlorinated biphenyls (PCBs) are currently regulated in food and feed within the European territory (EU 2017/644-771). The confirmatory methods of analysis for checking compliance with maximum levels (MLs) for these involve either the historically-established GC-magnetic sector high-resolution mass spectrometry (GC-HRMS) and, more recently, GC-triple quadrupole mass spectrometry operating in tandem mode (GC-QQQMS/MS). In this study, the performance of a novel triple quadrupole GC-QQQMS/MS system equipped with a programable temperature vaporization (PTV) injector was evaluated for the analysis of regulated PCDD/Fs and PCBs in food and feed. The MS analyzer was equipped with a titanium ionization chamber and a new short collision cell capable to accumulate and eject ions by means of very narrow pulses that allow to minimize the noise and to adapt accumulation times for sensitive multiple reaction monitoring (MRM). The analytical capability of the system was confronted by the strict requirements (selectivity, reproducibility, linearity, quant/qual MRM transitions, accuracy, robustness) set by the EU Regulation for a range of standards, quality control (QC) and food/feed samples. In this respect, the approach showed high precision (1.9&ndash 15% relative standard deviation (RSD) at low pg/µ L) and accuracy (> 80%, except for one hexa-CDD). The quantitative results were also compared to the most used GC-HRMS. In this case, comparable results in terms of single congener concentration basis and total toxic equivalent (TEQ) basis for PCDD/Fs and DL-PCBs were obtained for the QC samples analyzed. |
Databáze: | OpenAIRE |
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