Oligonucleotide hybridisations on glass supports: a novel linker for oligonucleotide synthesis and hybridisation properties of oligonucleotides synthesised in situ
| Autor: | Uwe Maskos, Edwin M. Southern |
|---|---|
| Přispěvatelé: | Department of Biochemistry [Oxford], University of Oxford [Oxford], U.M. was supported by the Maximilianeum, Munich, Germany, the Studienstiftung des deutschen Volkes, the Bayerische Begabtenforderung, and the Deutscher Akademischer Austauschdienst., We would like to thank Dr H. Blöcker for suggesting the 'trityl-on' assay to us and also for valuable comments on the manuscript. |
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Molecular Sequence Data
Oligonucleotides Sequence (biology) 02 engineering and technology Biology Oligonucleotide synthesis MESH: Base Sequence MESH: Chromatography Chemical synthesis Polyethylene Glycols MESH: Nucleic Acid Hybridization 03 medical and health sciences chemistry.chemical_compound Nucleic acid thermodynamics [SCCO]Cognitive science MESH: Oligonucleotides Genetics MESH: Silanes 030304 developmental biology 0303 health sciences Chromatography Silanes MESH: Molecular Sequence Data Base Sequence Oligonucleotide Nucleic Acid Hybridization 021001 nanoscience & nanotechnology Combinatorial chemistry Silane MESH: Glass chemistry Biochemistry MESH: Polyethylene Glycols Glass 0210 nano-technology Linker |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 1992, 20 (7), pp.1679-1684. ⟨10.1093/nar/20.7.1679⟩ |
| ISSN: | 0305-1048 1362-4962 |
| Popis: | International audience; A novel linker for the synthesis of oligonucleotides on a glass support is described. Oligonucleotides synthesised on the support remain tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions. These hybridizations were carried out with oligonucleotides synthesised on 'ballotini' solid sphere glass beads and microscope slides. The linker has a hexaethylene glycol spacer, bound to the glass via a glycidoxypropyl silane, terminating in a primary hydroxyl group that serves as starting point for automated or manual oligonucleotide synthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|