Generation of a homozygous ARX nuclear CFP (ARX) reporter human iPSC line (HMGUi001-A-4)
| Autor: | Noel Moya, Mostafa Bakhti, Julia Beckenbauer, Heiko Lickert, Alireza Shahryari, Ingo Burtscher |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
education.field_of_study Lineage (genetic) biology Karyotype Cell Biology General Medicine In vitro Cell biology ddc 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Histone lcsh:Biology (General) Aristaless related homeobox biology.protein Induced pluripotent stem cell education lcsh:QH301-705.5 Transcription factor 030217 neurology & neurosurgery Developmental Biology Progenitor |
| Zdroj: | Stem Cell Research, Vol 46, Iss, Pp 101874-(2020) |
| Popis: | The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARXnCFP/nCFP reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon+ α-like cells. Thus, ARXnCFP/nCFP iPSC line will provide a powerful tool to monitor human α-cell progenitor differentiation as well as ARX+ α-like cell function in vitro. |
| Databáze: | OpenAIRE |
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