Reduction of parasite levels in blood improves pregnancy outcome during experimentalTrypanosoma cruziinfection
| Autor: | C. D. Alba Soto, María Celia Fernández, S. M. Gonzalez Cappa, María Elisa Solana, Miriam Postan, Carolina Verónica Poncini |
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| Rok vydání: | 2009 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes Chagas disease Time Factors Placenta Trypanosoma cruzi Antibodies Protozoan Parasitemia Parasite load Andrology Interferon-gamma Mice Immune system Antigens CD Pregnancy parasitic diseases medicine Animals Chagas Disease Lectins C-Type Fetus biology Pregnancy Outcome biology.organism_classification medicine.disease Hyaluronan Receptors Infectious Diseases Gene Expression Regulation Nitroimidazoles Benznidazole Immunoglobulin G Pregnancy Complications Parasitic Immunology Gestation Female Animal Science and Zoology Parasitology Spleen medicine.drug |
| Zdroj: | Parasitology. 136:627-639 |
| ISSN: | 1469-8161 0031-1820 |
| Popis: | SUMMARYInfection with a myotropicTrypanosoma cruziclone induces maternal fertility failure. In the current work, we evaluated whether reduction of maternal parasitaemia before mating has beneficial effects on pregnancy outcome. Female mice were subjected to benznidazole (Bz) treatment after infection. On day 30 of therapy, mating was assessed and pregnancy outcome was determined on day 14 of gestation. Fetal resorptions diminished inT. cruzi-infected Bz-treated mice compared withT. cruzi-infected untreated mice. This was in agreement with the reduction in the blood/solid tissue parasite load and with the percentage of necrotic foci in placental samples fromT. cruzi-infected Bz-treated females. To study eventual changes in the immune homeostasis ofT. cruzi-infected Bz-treated mice, activation of the immune system was evaluated at the end of Bz therapy and before mating. We found specific IgG1reduction resulting in a predominance of specific IgG2a, reduced numbers of CD69+CD4+cells and diminished frequency and numbers of CD44+T cells. Concanavalin A-stimulated splenocytes fromT. cruzi-infected Bz-treated mice produced higher amounts of IFN-γ thanT. cruzi-infected untreated mice. These results indicate that reduction of maternal parasite load improves pregnancy outcome. These findings correlate with a favourable modulation of the immune response. |
| Databáze: | OpenAIRE |
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