Implication of glucocorticoid receptors in the stimulation of human glioma cell proliferation by dexamethasone
| Autor: | W. Sutanto, J C Stoof, M P van Waas, J J Heimans, C H Langeveld, E.R. de Kloet, J G Wolbers |
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| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Tetrazolium Salts Stimulation Biology Dexamethasone Cellular and Molecular Neuroscience chemistry.chemical_compound Receptors Glucocorticoid Glucocorticoid receptor Glioma Internal medicine Tumor Cells Cultured medicine Humans Receptor Cell growth Antiglucocorticoid medicine.disease Mifepristone Thiazoles Endocrinology chemistry Cell culture Cell Division medicine.drug |
| Zdroj: | Journal of Neuroscience Research. 31:524-531 |
| ISSN: | 1097-4547 0360-4012 |
| DOI: | 10.1002/jnr.490310316 |
| Popis: | Dexamethasone is frequently used in the therapy of brain tumor patients. We investigated the effect of dexamethasone on the proliferation of three short-term and four established human glioma cell lines in vitro, using a microculture tetrazolium assay to determine growth rates. In one short-term culture and in one established cell line dexamethasone consistently stimulated the proliferation in a concentration-dependent way. The proliferation was maximally enhanced at a concentration of approximately 0.1 microM. In these two cell lines a relatively high level of glucocorticoid receptors was present, whereas low levels of glucocorticoid receptors were found in the other cell lines. In addition, we demonstrated that the stimulatory effects of dexamethasone on the proliferation of the glioma cell lines can be antagonized by the antiglucocorticoid RU38486. The results demonstrate unequivocally that the glucocorticoid receptor plays a role in the growth stimulating effect of dexamethasone. |
| Databáze: | OpenAIRE |
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