Multiple-locus variable number of tandem repeat analysis as a tool for molecular epidemiology of botulism: The Italian experience
| Autor: | Silvia Fillo, Domenico Azarnia Tehran, Fabrizio Anniballi, Francesco Giordani, Giuseppina Mandarino, Bruna Auricchio, Dario De Medici, Florigio Lista, Enrica Di Stefano |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Prevalence Biology Multiple Loci VNTR Analysis medicine.disease_cause Microbiology 03 medical and health sciences Environmental health Genetics medicine Clostridium botulinum Cluster Analysis Humans Botulism Molecular Biology Ecology Evolution Behavior and Systematics Molecular Epidemiology Food poisoning Molecular epidemiology Outbreak medicine.disease 030104 developmental biology Infectious Diseases Italy Tandem Repeat Sequences Multilocus sequence typing Multilocus Sequence Typing |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 46 |
| ISSN: | 1567-7257 |
| Popis: | Clostridium botulinum is the bacterial agent of botulism, a rare but severe neuro-paralytic disease. Because of its high impact, in Italy botulism is monitored by an ad hoc surveillance system. The National Reference Centre for Botulism, as part of this system, collects and analyzes all demographic, epidemiologic, microbiological, and molecular data recovered during cases and/or outbreaks occurred in Italy. A panel of 312 C. botulinum strains belonging to group I were submitted to MLVA sub-typing. Strains, isolated from clinical specimens, food and environmental samples collected during the surveillance activities, were representative of all forms of botulism from all Italian regions. Through clustering analysis isolates were grouped into 12 main clusters. No regional or temporal clustering was detected, demonstrating the high heterogeneity of strains circulating in Italy. This study confirmed that MLVA is capable of sub-typing C. botulinum strains. Moreover, MLVA is effective at tracing and tracking the source of contamination and is helpful for the surveillance system in terms of planning and upgrading of procedures, activities and data collection forms. |
| Databáze: | OpenAIRE |
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