Survival of bacteria of laboratory animal origin on cage bedding and inactivation by hydrogen peroxide vapour
| Autor: | Christina Gougoula, Roland Schulze-Röbbecke, Eva Engelhardt, W. Peter M. Benten, Martin Sager, Laurentiu Benga |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Bedding 040301 veterinary sciences 030106 microbiology Biology Animal origin Microbiology 0403 veterinary science Mice 03 medical and health sciences chemistry.chemical_compound Animals Laboratory Escherichia coli Animals Food science Hydrogen peroxide Bacteria General Veterinary Bedding and Linens Hydrogen Peroxide 04 agricultural and veterinary sciences biology.organism_classification Housing Animal Disinfection chemistry Animal Science and Zoology Desiccation Cage |
| Zdroj: | Laboratory Animals. 51:412-421 |
| ISSN: | 1758-1117 0023-6772 |
| DOI: | 10.1177/0023677216675386 |
| Popis: | This study aims to determine the ability of laboratory animal bacteria to resist desiccation and inactivation by hydrogen peroxide vapour (HPV) on paper bedding pieces. Bedding pieces were saturated with bacterial suspensions in water or 2% (w/v) bovine serum albumin (BSA) in water, and held in a mouse facility. Viable counts showed variable survival rates over time for the bacterial species used ([ Pasteurella] pneumotropica, Muribacter muris, Pseudomonas aeruginosa, Acinetobacter redioresistens, Escherichia coli, Klebsiella oxytoca, Bordetella bronchiseptica, Bordetella hinzii, Enterococcus faecalis, β-haemolytic Streptococcus spp., Staphylococcus aureus and Staphylococcus xylosus). Overall, BSA increased bacterial survival in the bedding pieces. The survival rates of Bacillus safensis were not influenced by BSA but depended on sporulation. When bedding pieces and Petri dishes inoculated with E. coli, P. aeruginosa and S. aureus were subjected to HPV disinfection, all bacterial species on the bedding pieces inoculated with bacterial suspensions in water were readily inactivated. By contrast, S. aureus and P. aeruginosa, but not E. coli cells survived HPV treatment in high numbers when inoculated on bedding pieces as a BSA suspension. Notably, all three bacterial species were readily inactivated by HPV even in the presence of BSA when smeared on smooth surfaces. In conclusion, the suspension medium and the carrier can influence the environmental survival and susceptibility of bacterial species to HPV. Our results may help to develop standard protocols that can be used to ensure the microbiological quality of experimental rodent housing. |
| Databáze: | OpenAIRE |
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