Latent Infection and Reactivation of Human Herpesvirus 6 in Two Novel Myeloid Cell Lines
| Autor: | Eiji Sada, Masahiko Kaneko, Hideki Ohminami, Kohsuke Yanagisawa, Hidehisa Kohno, Shigeru Fujita, Shiro Bando, Masaki Yasukawa, Yoshihiro Yakushijin |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Myeloid Herpesvirus 6 Human viruses Immunology Biology Virus Replication medicine.disease_cause Polymerase Chain Reaction Biochemistry Herpesviridae Cytopathogenic Effect Viral Antigen Leukemia Myelogenous Chronic BCR-ABL Positive Tumor Cells Cultured medicine Humans Antigens Viral Cytopathic effect Syncytium virus diseases Cell Biology Hematology biochemical phenomena metabolism and nutrition Hematopoietic Stem Cells medicine.disease Virology Virus Latency Haematopoiesis medicine.anatomical_structure Cell culture DNA Viral Neoplastic Stem Cells Tetradecanoylphorbol Acetate Virus Activation Blast Crisis Chronic myelogenous leukemia |
| Zdroj: | Blood. 93:991-999 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | It has been reported that reactivation of human herpesvirus-6 (HHV-6) causes a failure of hematopoiesis. To clarify the mechanisms of bone marrow suppression induced by HHV-6 infection, it is necessary to establish an in vitro model of HHV-6 infection in hematopoietic progenitor cells. We have established two novel Philadelphia chromosome–positive myeloid cell lines, SAS413 and SAS527, which possess different hematologic characteristics and show distinct susceptibility to infection by HHV-6, from a patient with blast crisis of chronic myelogenous leukemia (CML). HHV-6 subgroup A (HHV-6A) showed marked replication in SAS413, forming syncytia and inducing cell lysis in short-term culture. On the other hand, HHV-6A–inoculated SAS527 continued to proliferate without cell lysis and only a few cells showed HHV-6 antigen expression. In contrast to HHV-6A infection, inoculation with HHV-6 subgroup B (HHV-6B) did not induce any cytopathic effect (CPE) or viral antigen expression in either of the cell lines. Although HHV-6B replication was undetectable, the presence of the HHV-6 genome in both cell lines was shown by polymerase chain reaction (PCR) during culture for more than 10 months, suggesting that HHV-6B latently infected SAS413 and SAS527. Phorbol ester treatment of SAS527 latently infected with HHV-6B resulted in reactivation of HHV-6, as shown by the appearance of a CPE, positive reactivity for the HHV-6 antigen, and isolation of infectious HHV-6. These novel cell lines should be useful for studying the mechanisms of HHV-6–induced hematopoietic failure and HHV-6 latency and reactivation, as well as differentiation, of the myeloid cell lineage. |
| Databáze: | OpenAIRE |
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