Heterologous expression and functional analysis of the gene cluster for the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1

Autor:	Jiro Nakayama, Youhei Kanemasa, Hanako Koga, Kenji Sonomoto, Jun Ichi Nagao, Ken Ichi Okuda, Yuji Aso, Toshihiro Sashihara
Rok vydání:	2004
Předmět:	biology Lactococcus lactis Heterologous Bioengineering Lantibiotics biology.organism_classification Applied Microbiology and Biotechnology law.invention Microbiology Bacteriocin law Staphylococcus warneri Gene cluster Recombinant DNA Heterologous expression Biotechnology
Zdroj:	Journal of bioscience and bioengineering. 98(6)
ISSN:	1389-1723
Popis:	Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. The gene cluster of nukacin ISK-1 consists of at least nukAMTFEG, ORF1 and ORF7. In this study, we demonstrated the heterologous production of nukacin ISK-1 in Lactococcus lactis by the artificial polycistronic expression of nukAMTFEG-ORF7 under the control of the nisin-controlled expression (NICE) system. Consequently, the recombinant L. lactis showed antimicrobial activity. Mass analysis clarified the presence of nukacin ISK-1 produced in the culture supernatant. These results suggested that the recombinant L. lactis produced nukacin ISK-1 heterologously. Inactivation of nukA, -M or -T resulted in the complete loss of the nukacin ISK-1 production phenotype. This finding suggested that nukAMT are indispensably associated with the biosynthesis of nukacin ISK-1. To our knowledge, this is the first report of the heterologous production of lantibiotic using the NICE system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ffa15a7b8052a18c667d10d8fb75c44 https://pubmed.ncbi.nlm.nih.gov/16233732 Zobrazit plný text záznamu Full Text from ScienceDirect