Speed of in vitro embryo development affects the likelihood of foaling and the foal sex ratio
| Autor: | Claes, A, Cuervo-Arango, J, Colleoni, S, Lazzari, G, Galli, C, Stout, T A, Voortplanting paard, dES/dFAH FR, CS_Fertility |
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| Přispěvatelé: | Voortplanting paard, dES/dFAH FR, CS_Fertility |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Time Factors medicine.medical_treatment animal diseases Reproductive technology Cryopreservation Intracytoplasmic sperm injection 0403 veterinary science Embryo Culture Techniques 0302 clinical medicine Endocrinology reproductive and urinary physiology media_common equine 030219 obstetrics & reproductive medicine biology Embryo 04 agricultural and veterinary sciences medicine.anatomical_structure Foal embryonic structures Female intracytoplasmic sperm injection (ICSI) pregnancy Live Birth Biotechnology 040301 veterinary sciences media_common.quotation_subject in vitro embryo production (IVEP) Andrology 03 medical and health sciences biology.animal Genetics medicine Animals Blastocyst Horses Sex Ratio Sperm Injections Intracytoplasmic Molecular Biology Ovulation Retrospective Studies Pregnancy urogenital system medicine.disease Embryo Transfer In Vitro Oocyte Maturation Techniques Reproductive Medicine Animals Newborn Animal Science and Zoology Developmental Biology |
| Zdroj: | Reproduction, Fertility and Development, 32(5), 468. CSIRO |
| ISSN: | 1031-3613 |
| Popis: | The success of invitro embryo production (IVEP) in horses has increased considerably during recent years, but little is known about the effect of the speed of invitro embryo development. Blastocysts (n=390) were produced by intracytoplasmic sperm injection of IVM oocytes from warmblood mares, cryopreserved, thawed and transferred into recipient mares on Days 3, 4, 5 or 6 after ovulation. The time required for invitro-produced (IVP) embryos to reach the blastocyst stage was recorded (Day 7 vs Day 8). The likelihood of foaling was affected by the speed of invitro embryo development and recipient day after ovulation at transfer. The odds ratio for foaling was ~0.63 for transfer of Day 8 (46%) compared with Day 7 (56%) IVP blastocysts. The highest likelihood of pregnancy (72%) and foaling (60%) was observed when IVP blastocysts were transferred to recipient mares on Day 4 after ovulation. Finally, the sex (colt:filly) ratio was higher after transfer of Day 7 (71%:29%) than Day 8 (54%:46%) IVP blastocysts, suggesting that the speed of embryo development is sex dependent. In conclusion, the speed of invitro embryo development in our IVEP system affects the likelihood of foaling and the sex of the foal. |
| Databáze: | OpenAIRE |
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