Parainfluenza virus type 1 induces epithelial IL-8 production via p38-MAPK signalling
| Autor: | Carlos Cabello Gutiérrez, Leticia Valle Peralta, Miguel Ángel Galván Morales, María Eugenia Manjarrez Zavala, Fidencio Mejía Nepomuceno, Elba Valencia Maqueda |
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| Rok vydání: | 2013 |
| Předmět: |
MAPK/ERK pathway
lcsh:Immunologic diseases. Allergy Article Subject Ultraviolet Rays p38 mitogen-activated protein kinases Immunology Gene Expression Biology Virus Replication p38 Mitogen-Activated Protein Kinases Epithelium Cell Line Western blot medicine Immunology and Allergy Humans Phosphorylation Protein Kinase Inhibitors medicine.diagnostic_test Kinase Interleukin-8 JNK Mitogen-Activated Protein Kinases Epithelial Cells General Medicine Molecular biology Cell biology Parainfluenza Virus 1 Human Viral replication Cell culture Virus Inactivation Signal transduction lcsh:RC581-607 Signal Transduction Research Article |
| Zdroj: | Journal of Immunology Research Journal of Immunology Research, Vol 2014 (2014) |
| ISSN: | 2314-7156 |
| Popis: | Human parainfluenza virus type 1 (HPIV-1) is the most common cause of croup in infants. The aim of this study was to describe molecular mechanisms associated with IL-8 production during HPIV-1 infection and the role of viral replication in MAPK synthesis and activation. Anin vitromodel of HPIV-1 infection in the HEp-2 and A549 cell lines was used; a kinetic-based ELISA for IL-8 detection was also used, phosphorylation of the mitogen-activated protein kinases (MAPKs) was identified by Western blot analysis, and specific inhibitors for each kinase were used to identify which MAPK was involved. Inactivated viruses were used to assess whether viral replication is required for IL-8 production. Results revealed a gradual increase in IL-8 production at different selected times, when phosphorylation of MAPK was detected. The secretion of IL-8 in the two cell lines infected with the HPIV-1 is related to the phosphorylation of the MAPK as well as viral replication. Inhibition of p38 suppressed the secretion of IL-8 in the HEp-2 cells. No kinase activation was observed when viruses were inactivated. |
| Databáze: | OpenAIRE |
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