c-Fos is necessary for HGF-mediated gene regulation and cell migration in Schwann cells

Autor: Junghun Lee, Kyeong Ryang Ko, Boram Nho, Sun-Young Kim
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
MAPK/ERK pathway
Indoles
Nerve Crush
Primary Cell Culture
Biophysics
CREB
Leukemia Inhibitory Factor
Biochemistry
Proto-Oncogene Proteins c-myc
Mice
03 medical and health sciences
0302 clinical medicine
Cell Movement
Nitriles
Gene expression
Butadienes
Glial cell line-derived neurotrophic factor
medicine
Animals
Glial Cell Line-Derived Neurotrophic Factor
Sulfones
RNA
Small Interfering
Molecular Biology
Regulation of gene expression
biology
Hepatocyte Growth Factor
Chemistry
Cell migration
Cell Biology
Proto-Oncogene Proteins c-met
Sciatic Nerve
Recombinant Proteins
Cell biology
Mice
Inbred C57BL
Transcription Factor AP-1
030104 developmental biology
Gene Expression Regulation
nervous system
biology.protein
Hepatocyte growth factor
Schwann Cells
Proto-Oncogene Proteins c-fos
030217 neurology & neurosurgery
Signal Transduction
Neurotrophin
medicine.drug
Zdroj: Biochemical and Biophysical Research Communications. 503:2855-2860
ISSN: 0006-291X
Popis: We previously reported that the expression of hepatocyte growth factor (HGF) was highly induced after peripheral nerve damage, and that c-Fos is one of many cellular genes whose expressions are affected by the increased level of HGF[1]. c-Fos is an important component of AP-1 heterodimer, but its role has not been clearly understood in the context of HGF and Schwann cells (SCs). In this study, we investigated the relationship between HGF and c-Fos. First, it was confirmed that the c-Fos was increased in SCs after nerve injury, while this effect abrogated by PHA-665752, an inhibitor of c-met receptor. When primary SCs were treated with recombinant HGF protein, c-Fos expression was regulated in a typical quick, transient fashion at both RNA and proteins levels. HGF-mediated induction of c-Fos expression was highly suppressed by specific inhibitors of ERK and CREB, respectively. The knock down of c-Fos expression by siRNA almost completely blocked various HGF-mediated effects in SCs, such as induction of gene expression of GDNF, LIF, and c-Myc, and migration of SCs, indicating that c-Fos might play a key role in HGF effects. Taken together, our results suggested that c-Fos plays a key role(s) in HGF-mediated effects on neurotrophic genes and cell migration.
Databáze: OpenAIRE
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