Oxidative DNA and RNA damage and their prognostic values during Salmonella enteritidis-induced intestinal infection in rats

Autor:	Ran Zhang, Chuan-Bao Li, Jihong Hu, Zhe-Li Jiang, Jingjing Nie, Yaya Pian, Qing-Hua Weng, Jian-Ping Cai, Zhen-He Wang, Zhenxiang Gao, Meng-Ming Xia
Rok vydání:	2018
Předmět:	0301 basic medicine medicine.drug_class Salmonella enteritidis Antibiotics Spleen Urine medicine.disease_cause Infections Biochemistry Microbiology 03 medical and health sciences Predictive Value of Tests medicine Animals Humans heterocyclic compounds Intestinal Mucosa Norfloxacin business.industry General Medicine Small intestine Rats Oxidative Stress 030104 developmental biology medicine.anatomical_structure Liver Biomarker (medicine) RNA business Oxidation-Reduction Oxidative stress medicine.drug DNA Damage
Zdroj:	Free radical research. 52(9)
ISSN:	1029-2470
Popis:	Emerging evidence suggests that microbial pathogens may induce oxidative stress in infected hosts. The aim of the present study was to investigate the relationship between changes in oxidative stress and intestinal infection with and without antibiotic treatment in animal models. Sprague-Dawley (SD) rats were divided into three groups: rats infected with Salmonella enterica serovar Enteritidis (S. enteritidis), rats infected with S. enteritidis followed by norfloxacin treatment, and the control group. To evaluate oxidative stress changes, levels of 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) and 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxo-dGsn), which represented oxidative damage to RNA and DNA, respectively, were analysed in urine and tissue samples. In urine, the level of 8-oxo-Gsn increased significantly after oral exposure to S. enteritidis (p ≤ 0.001) and returned to baseline after recovery. Notably, norfloxacin treatment decreased the level of 8-oxo-Gsn in urine significantly (p = 0.001). Changes of 8-oxo-Gsn measured in tissues from the small intestine, colon, liver and spleen were consistent with 8-oxo-Gsn measured in urine. Our study suggested that 8-oxo-Gsn in urine may serve as a highly sensitive biomarker for evaluating the severity of S. enteritidis infection and the effectiveness of antibiotic treatment against infection.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ff353c2ed84e1b58d27ca3bd30b2df3 https://pubmed.ncbi.nlm.nih.gov/30422023 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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