Metabolomic response to coffee consumption: application to a three-stage clinical trial
| Autor: | Christian Herder, Jaakko Tuomilehto, Iris Erlund, G. A. Michelotti, Marilyn C. Cornelis, Johan A. Westerhuis |
|---|---|
| Přispěvatelé: | Biosystems Data Analysis (SILS, FNWI) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Metabolite Gut flora Benzoates Coffee Xanthine 03 medical and health sciences chemistry.chemical_compound Metabolomics Internal Medicine Metabolome Humans Medicine Single-Blind Method Food science biology Fatty acid metabolism business.industry Microbiota Phytosterol Fatty Acids Fasting Metabolism biology.organism_classification 030104 developmental biology chemistry Steroids Caffeine business Biomarkers Metabolic Networks and Pathways Endocannabinoids |
| Zdroj: | Journal of internal medicine, 283(6), 544-557. Wiley-Blackwell |
| ISSN: | 0954-6820 |
| Popis: | BackgroundCoffee is widely consumed and contains many bioactive compounds, any of which may impact pathways related to disease development. ObjectiveTo identify individual metabolite changes in response to coffee. MethodsWe profiled the metabolome of fasting serum samples collected from a previously reported single‐blinded, three‐stage clinical trial. Forty‐seven habitual coffee consumers refrained from drinking coffee for 1 month, consumed four cups of coffee/day in the second month and eight cups/day in the third month. Samples collected after each coffee stage were subject to nontargeted metabolomic profiling using UPLC‐ESI‐MS/MS. A total of 733 metabolites were included for univariate and multivariate analyses. ResultsA total of 115 metabolites were significantly associated with coffee intake (P < 0.05 and Q < 0.05). Eighty‐two were of known identity and mapped to one of 33 predefined biological pathways. We observed a significant enrichment of metabolite members of five pathways (P < 0.05): (i) xanthine metabolism: includes caffeine metabolites, (ii) benzoate metabolism: reflects polyphenol metabolite products of gut microbiota metabolism, (iii) steroid: novel but may reflect phytosterol content of coffee, (iv) fatty acid metabolism (acylcholine): novel link to coffee and (v) endocannabinoid: novel link to coffee. ConclusionsThe novel metabolites and candidate pathways we have identified may provide new insight into the mechanisms by which coffee may be exerting its health effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text